Brilliant blue G protects against brain and liver tissue damage during systemic endotoxemia in rats treated with lipopolysaccharide

In this study, the effect of brilliant blue G on serum oxidative stress and brain and liver tissue damage was studied in rats with lipopolysaccharide (LPS)-induced systemic inflammation. Rats were treated with intraperitoneal (i.p.) injection ofEscherichia coli LPS (300  μg/kg) alone or together with brilliant blue G at 5 or 10 mg/kg and euthanized 4 h thereafter. Serum malondialdehyde, nitric oxide, paraoxonase 1 (PON-1) activity, cholinesterase activity, and glucose were determined. In addition, brain and liver histopathology, and caspase-3 and glial fibrilla ry acidic protein (GFAP) immunostaining were done. Results indicated that LPS produced a significant elevation in serum malondialdehyde and nitric oxide concentrations along with markedly decreased PON-1 activity and glucose. LPS caused neuronal degeneration in the cerebral cortex and hippocampus, i ncreased caspase-3, and decreased GFAP immunostaining in the cerebral cortex. Vacuolar degeneration and inflammation were observed in the liver of LPS-treated rats. The administration of brilliant blue G decreased serum malondialdehyde by 34.5–35.2% and nitric oxide concentrations by 27.4–35.6%, respectively, while increasing PON-1 activity by 46.3–86.7% and serum glucose level by 24.8%. Moreover, brilliant blue G inhibited serum cholinesterase activity by 38.1–42% compared with the LPS control group. Brilliant blue G attenuated the neuronal degeneration, the increase in caspase-3 acti vity, and the decrease in ...
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research