Finding Sarcocystis spp. on the Tioman Island: 28S rRNA gene next-generation sequencing reveals nine new Sarcocystis species.

This study aimed to improve the detection methods for Sarcocystis, in order to have a clearer picture of the true diversity of Sarcocystis species in Tioman. A new primer set (28S R7F-28S R8 Deg R) was designed to amplify the 28S rRNA gene of Sarcocystis. Subsequently, Sarcocystidae was detected in 65.6% (21/32) of water samples and 28% (7/25) of soil samples acquired between 2014 and 2015 from Tioman. Next-generation sequencing (NGS) on 18 of the positive samples was then performed using amplicons generated from the same primer set. This yielded 53 potentially unique Sarcocystidae sequences (290 bp), of which nine of the most abundant, prevalent and unique sequences were named herein. In contrast, NGS of the 18S rRNA gene V9 hypervariable region of 10 selected samples detected only two Sarcocystis species (160 bp). S. mantioni was the most ubiquitous sequence found in this study. PMID: 31095517 [PubMed - in process]
Source: Journal of Water and Health - Category: Environmental Health Tags: J Water Health Source Type: research

Related Links:

Publication date: Available online 20 June 2019Source: Preventive Veterinary MedicineAuthor(s): Jeiczon Jaimes-Dueñez, Carolina Zapata-Zapata, Omar Triana-Chávez, Ana M. Mejía-JaramilloAbstractSurra is a zoonotic disease caused by Trypanosoma evansi, affecting the health and production of the livestock significantly. There are several methods to diagnose this disease, which have different principles, sensitivity, and specificity. Among them, the serological techniques using T. evansi as antigen are powerful tools for its epidemiological surveillance. However, they are poorly used due to inefficient in ...
Source: Preventive Veterinary Medicine - Category: Veterinary Research Source Type: research
(University of Pennsylvania) The intestinal parasite Cryptosporidium causes frequent outbreaks in the US, and has been historically difficult to study. A novel model of infection from the University of Pennsylvania serves as a new tool to pursue a vaccine.
Source: EurekAlert! - Infectious and Emerging Diseases - Category: Infectious Diseases Source Type: news
CONCLUSION: Metronidazole, tinidazole, and nitazoxanide are the drugs of choice. Resistance to common antigiardial drugs has increased in recent years. As such, the search for new molecular targets for antigiardial drugs is urgently needed. In general, treatment of asymptomatic carriers is not recommended. Purification of water supply is an important preventive measure. PMID: 31210116 [PubMed - as supplied by publisher]
Source: Recent Patents on Inflammation and Allergy Drug Discovery - Category: Allergy & Immunology Tags: Recent Pat Inflamm Allergy Drug Discov Source Type: research
We report the discovery of Trichinella pseudospiralis (T4), a non-encapsulated species, in a wolverine (Gulo gulo) from the Northwest Territories of Canada. This parasite has been previously reported elsewhere from both mammals and carnivorous birds, but our findings represent new host and geographic records for T. pseudospiralis. Multiplex PCR and sequencing of fragments of Cytochrome Oxidase Subunit I (COI) and D3 rDNA confirmed the identification. Phylogenetically, Canadian isolates linked with each other and others derived from Palearctic or Neotropical regions, but not elsewhere in the Nearctic (continental USA). We s...
Source: International Journal for Parasitology: Parasites and Wildlife - Category: Parasitology Source Type: research
ConclusionsIn this paper we discuss the current state of the results and draw on evidences from malaria, dengue and other vector-borne diseases to illustrate the state of current thinking and outline the need for further research to inform our predictions and response.
Source: Infectious Diseases of Poverty - Category: Infectious Diseases Source Type: research
o RMB Abstract Giardia and Cryptosporidium have caused numerous outbreaks of diarrhea as a result of the ingestion of water contaminated with sewage. In Brazil, the efficiency of Giardia and Cryptosporidium removal by combined fixed-film systems has rarely been studied. The aims of the present study were therefore to verify the removal efficiency of Giardia and Cryptosporidium by a combined system (anaerobic/anoxic filter and aerated submerged biofilter) and to perform the genetic characterization of these parasites. The (oo)cysts were detected by centrifuge concentration and membrane filtration from raw sewage, e...
Source: Environmental Science and Pollution Research International - Category: Environmental Health Authors: Tags: Environ Sci Pollut Res Int Source Type: research
by Laura Botana, Ana Victoria Ibarra-Meneses, Carmen S ánchez, Alicia Castro, Juan Victor San Martin, Laura Molina, Jose Manuel Ruiz-Giardin, Eugenia Carrillo, Javier Moreno Concomitant infection with human immunodeficiency virus (HIV) and theLeishmania parasite is a growing public health problem, the result of the former spreading to areas where the latter is endemic.Leishmania infection is usually asymptomatic in immunocompetent individuals, but the proportion of HIV+ individuals in contact with the parasite who remain asymptomatic is not known. The aim of the present work was to examine the use of cytokine relea...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
Abstract The increased rate of outbreaks of infectious diseases in ecosystems is a dramatic consequence of global change, particularly when outbreaks affect important resources such as freshwater fish. However, the links between disease-inducing epizootics and widespread human impacts, including nutrient pollution and high water conductivity, in freshwater organisms are largely unexplored. We used data from extensive surveys in northeastern Spain (99,700 km2, 15 river catchments, n = 530 sites) to explore the environmental factors that singly, or in combination, are likely to influence the occurrence of the invasi...
Source: International Journal for Parasitology - Category: Parasitology Authors: Tags: Int J Parasitol Source Type: research
Authors: Wood M, Simmonds L, MacAdam J, Hassard F, Jarvis P, Chalmers RM Abstract Most commercial swimming pools use pressurised filters, typically containing sand media, to remove suspended solids as part of the water treatment process designed to keep water attractive, clean and safe. The accidental release of faecal material by bathers presents a poorly quantified risk to the safety of swimmers using the pool. The water treatment process usually includes a combination of maintaining a residual concentration of an appropriate biocide in the pool together with filtration to physically remove particles, including m...
Source: Journal of Water and Health - Category: Environmental Health Tags: J Water Health Source Type: research
This report summarizes confirmed malaria cases in persons with onset of illness in 2016 and summarizes trends in previous years. DESCRIPTION OF SYSTEM: Malaria cases diagnosed by blood film microscopy, polymerase chain reaction, or rapid diagnostic tests are reported to local and state health departments by health care providers or laboratory staff members. Case investigations are conducted by local and state health departments, and reports are transmitted to CDC through the National Malaria Surveillance System (NMSS), the National Notifiable Diseases Surveillance System (NNDSS), or direct CDC consultations. CDC refer...
Source: MMWR Surveill Summ - Category: Epidemiology Authors: Tags: MMWR Surveill Summ Source Type: research
More News: Environmental Health | Genetics | Malaysia Health | Outbreaks | Parasitic Diseases | Parasitology | Study