PAQR3 regulates phosphorylation of FoxO1 in insulin-resistant HepG2 cells via NF- κB signaling pathway.
In this study, we aimed to investigate whether PAQR3 regulates phosphorylation of FoxO1 via NF-κB pathway in palmitic acid (PA)-induced insulin-resistant HepG2 cells, thereby causing glucose and lipid metabolism disorders. We found that PA stimulation and PAQR3 overexpression decreased the phosphorylation of FoxO1 and the expressions of glucokinase (GCK) and low density lipoprotein receptor (LDLR), in addition, promoted the nuclear accumulation of NF-κB. Inhibition of NF-κB pathway increased the phosphorylation of FoxO1 and the expressions of GCK and LDLR which were downregulated by PA stimulation and PAQR3 overexpression. Taken together, in PA-induced insulin-resistant HepG2 cells, PAQR3 might regulate the phosphorylation of FoxO1 and the expressions of GCK and LDLR through NF-κB pathway, thereby regulating the glucose and lipid metabolism disorders induced by insulin resistance.
PMID: 31095939 [PubMed - as supplied by publisher]
Source: Experimental Cell Research - Category: Cytology Authors: Chen L, Sun X, Xiao H, Xu F, Yang Y, Lin Z, Chen Z, Quan S, Huang H Tags: Exp Cell Res Source Type: research
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