Determination of benzonatate and its metabolite in human plasma by HPLC–MS/MS: A preliminary pharmacokinetic study in healthy Chinese volunteers after oral administration of benzonatate soft capsule
In this study, two sensitive high-performance liquid chromatography-tandem mass spectrometry (HPLC–MS/MS) methods were developed and fully validated for the determination of the original 4-(butylamino)benzoic acid (method B) and total 4-(butylamino)benzoic acid (containing the original 4-(butylamino)benzoic acid and 4-(butylamino)benzoic acid converted from benzonatate after collection, method A). For both methods, one-step protein precipitation by methanol was performed to extract analytes from the plasma samples. Chromatographic separation was done on an InfinityLab Poroshell 120 Phenyl Hexyl column (2.1 mm × 50 mm, 2.7 μm, Agilent) with initial mobile phase consisting of 5 mM ammonium acetate containing 0.3% formic acid and acetonitrile (60:40, v/v) at a flow rate of 0.3 mL/min. Quantification was achieved by multiple reaction monitoring (MRM) in electron spray ionization (ESI) positive mode with the transitions of m/z 194.2→138.1 and 515.3→497.3 for 4-(butylamino)benzoic acid and telmisartan (the internal standard), respectively. The two methods exhibited good linearity over the concentration range of 10–10000 ng/mL. Both of the methods were successfully applied to the preliminary pharmacokinetic study in healthy Chinese volunteers after oral administration of benzonatate soft capsule at a single dose of 100 mg. The results showed that 4-(butylamino)benzoic acid and benzonatate w...
ConclusionsCompressed sensing reduced the acquisition time of conventional MR imaging of the ankle by 20% without decreasing diagnostic image quality, SNR and CNR.
Hilary Nickols, MD, PhDFrom time to time, I feature neuropathologists who exhibit talents beyond the strict confines of neuropathology. For example, I recently features the inimitableMark Cohen and his prodigious classical guitar skills. I discovered another neuropathologist/artist during the recent annual meeting of the American Association of Neuropathologists: Hilary Nickols, MD, PhD, of Norton Healthcare in Louisville, Kentucky. Dr. Nickols shared with me her detailed drawing of a craniotomy surgical field which she recently witnessed during a visit to the operating room.The scene in the operating room, including ...
Conclusion. This kind of procedure should be kept in the therapeutic armament in the current setting of costly targeted systemic treatments of spondyloarthritis. However, definition of a clear position in the treatment strategy needs further well conducted studies.
ConclusionsPamidronate is a safe and efficient method of CRMO therapy, particularly in cases refractory to NSAIDs treatment. Treatment with pamidronate provides both symptomatic relief as well as normalisation of bone morphology.
Publication date: Available online 16 June 2019Source: Joint Bone SpineAuthor(s): Pierre Letellier, Florian Bailly, Marina Assadourian, Antoine Potel, Violaine Foltz, Sophia Ascione, Laetitia Morardet, Myrianne Le Ralle, Bruno Fautrel, Arnaud Dupeyron, Nada Ibrahim-Nasser, Isabelle Griffoul-Espitalier, Bernard Duplan, Johann Beaudreuil, Laure Gossec
Inducing seizures with cortical stimulation accurately identifies the target area for resection and significantly reduces hospital stays, a new study shows.Medscape Medical News
Cochlear Limited has received U.S. Food and Drug Administration (FDA) clearance...Read more on AuntMinnie.comRelated Reading: FDA OKs new MRI-conditional cochlear implant
Pfizer has agreed to buy Array Biopharma in a $10.64 billion cash deal.
As researchers continue to hunt for ways to treat or prevent Alzheimer ’s disease, one study has shown promise in slowing the progression of the disease.
The company started with fewer than 20 employees in 2017.