Molecular characterization of highly pathogenic Eimeria species among beef cattle on Java Island, Indonesia

Publication date: Available online 17 May 2019Source: Parasitology InternationalAuthor(s): Fitrine Ekawasti, Wisnu Nurcahyo, April Hari Wardhana, Tomoyuki Shibahara, Masaharu Tokoro, Kazumi Sasai, Makoto MatsubayashiAbstractGastrointestinal parasites including Eimeria spp. are known to affect domestic animal productivity causing watery or lethal bloody diarrhea. However, there are few reports on the detailed distribution of bovine Eimeria spp. in cattle, particularly in developing tropical and sub-tropical areas. Using a total of 289 fecal samples collected from beef cattle on Java Island, one of the five main islands of Indonesia, fecal examinations by the Whitlock and sugar flotation methods and molecular surveys were conducted to reveal the prevalence of 6 Eimeria spp. As a result of morphological screening using Whitlock methods and sugar flotation, Eimeria spp. prevalences of 9.4% and 52.3% were confirmed, respectively. The prevalence was higher in younger cattle [1 year (63.9%), 1–2 years (75.0%) and more than in 2 year old cattle (28.6%)]. The prevalences of identified species were as follows: 10.4% for E. bovis, 2.8% for E. ellipsoidalis, 2.1% for E. alabamensis, 1.4% for E. zuernii, 1.1% for E. auburnensis, and 0.4% for E. cylindrica. Moreover, prevalences of 12.8% for Strongyloides papillosus, 7.3% for Trichuris globulosa, and 0.3% for Capillaria bovis were detected. Although the average number of oocysts per gram of feces was <100 among the positive sam...
Source: Parasitology International - Category: Parasitology Source Type: research