Stress granules are formed in renal proximal tubular cells during metabolic stress and ischemic injury for cell survival.

Stress granules are formed in renal proximal tubular cells during metabolic stress and ischemic injury for cell survival. Am J Physiol Renal Physiol. 2019 May 15;: Authors: Wang S, Kwon SH, Su Y, Dong Z Abstract Stress granule (SG) is a type of cytoplasmic structures formed in eukaryotic cells upon cell stress, which mainly contains RNA-binding proteins and RNAs. The formation of SG is generally regarded as a mechanism for cells to survive a harsh insult. However, little is known about SG formation and function in kidneys. To address this, we applied different kinds of stressors to cultured proximal tubular cells as well as a short period of ischemia/reperfusion to mouse kidneys. It was found that glycolytic inhibitors such as 2DG (2-deoxy-D-glucose) and 3PO (3-(3-pyridinyl)-1-(4-pyridinyl)-2-propen-1-one) induced SG formation within 30 minutes in these cells. Similarly, SGs were induced by inhibitors of mitochondrial respiration such as sodium azide and CCCP (carbonyl cyanide m-chlorophenyl hydrazone). Renal ischemia/reperfusion induced SG formation in the cells of proximal tubules. To test the role of SGs, we stably knocked down G3bp1, a SG core protein, in renal tubular cells by shRNA viral transduction. As expected, knockdown of G3bp1 largely disrupted the assembly of SG. After azide or cisplatin treatment, more dead cells were found in knockdown cells in comparison to controls, accompanied by increases in cleaved/active caspase-...
Source: Am J Physiol Renal P... - Category: Urology & Nephrology Authors: Tags: Am J Physiol Renal Physiol Source Type: research