Facile immobilization of Trametes versicolor laccase on highly monodisperse superparamagnetic iron oxide nanoparticles

Publication date: Available online 10 May 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Claudia Iriarte-Mesa, Sergio Díaz-Castañón, Darío G. AbradeloAbstractHypothesisThe development of enzymatic conjugates with industrial applications require approaches with good scalability and batch-to-batch reproducibility. Hereof, nearly monodisperse iron oxide nanoparticles can be synthesized by thermal decomposition with high yields. A mixture of gallic and polyacrylic acid is used for the direct water transfer and later immobilization of laccase (Trametes versicolor).ExperimentsNanoparticles were synthesized by thermal decomposition (13.1 nm by TEM, 50 nm by DLS) and later transferred to water by a ligand exchange method with polyacrylic acid and a polyacrylic acid/gallic acid mixture. Laccase was immobilized on water dispersions of both nanoparticles via a carbodiimide coupling.FindingsThe nanoparticles exhibited superparamagnetic behavior with insignificant values of iHc. The presence of gallic acid hindered the formation of multiple polyacrylic acid layers, therefore improving the colloidal stability of the nanoparticles (100 nm by DLS) after weeks of storage. Nanoparticles containing only polyacrylic acid showed poor activity (60 % loading, 4.5 % activity), while nanoparticles with both polyacrylic and gallic acids showed enzymatic activity values 4.4 times higher than the free enzyme (13 % loading, 57 % activity). The nanoparticles improved the storage st...
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research