Identification of a regeneration-organizing cell in the Xenopus tail
Unlike mammals, Xenopus laevis tadpoles have a high regenerative potential. To characterize this regenerative response, we performed single-cell RNA sequencing after tail amputation. By comparing naturally occurring regeneration-competent and -incompetent tadpoles, we identified a previously unrecognized cell type, which we term the regeneration-organizing cell (ROC). ROCs are present in the epidermis during normal tail development and specifically relocalize to the amputation plane of regeneration-competent tadpoles, forming the wound epidermis. Genetic ablation or manual removal of ROCs blocks regeneration, whereas transplantation of ROC-containing grafts induces ectopic outgrowths in early embryos. Transcriptional profiling revealed that ROCs secrete ligands associated with key regenerative pathways, signaling to progenitors to reconstitute lost tissue. These findings reveal the cellular mechanism through which ROCs form the wound epidermis and ensure successful regeneration.
A new optimized method for revascularizing bioengineered livers provides up to 15 days of continuous perfusion after transplantation into immunosuppressed pigs.Reuters Health Information
Publication date: Available online 19 October 2019Source: Journal of the Formosan Medical AssociationAuthor(s): Jheng-Zong Tien, Chia-Huei Chou, Mao-Wang Ho, Tzu-Ting Chen
Publication date: Available online 18 October 2019Source: Best Practice &Research Clinical HaematologyAuthor(s): Eunice S. WangAbstractMutations of FLT3 occur in around a third of acute myeloid leukemia (AML) patients and are associated with poor outcomes. Multiple targeted tyrosine kinase inhibitors (TKI) have been developed with different selectivity and potency for FLT3 mutant clones. Indications for which FLT3-inhibitor to use depend on the clinical setting and disease status. Patients with relapsed or refractory AML benefit from a different TKI than those with de novo AML or following stem cell transplant. Moreove...
ConclusionPresumed higher anti-leukemic activity of RIC versus NMA conditioning did not translate into better outcomes after alloHSCT, but better remission status prior to conditioning did. Effective pathway inhibitor-based salvage therapies combined with NMA conditioning might thus represent the most attractive contemporary approach for alloHSCT for patients with CLL.
This study analyzed PCT and CRP levels of 214 pediatric patients with a median age of 8.5 years (0.4–17.8 years) undergoing allogeneic HSCT with respect to major TRAE.Results26 patients (12.1%) did not experience TRAE (control group), and 188 (87.9%) experienced median 2 (range 1 –4) TRAE. Median CRP and PCT were highly and significantly increased during sepsis/SIRS and bacteremia (17.24 mg/dl | 6.30 ng/ml;p
Conclusion: Our study is designed to highlight how fibrofatty changes are minimal using CMRI in the pediatric population and how this can be a valuable tool to provide an additional method of diagnosis. PMID: 31625461 [PubMed - as supplied by publisher]
Authors: Sedano R, Castro L, Venegas M, Miranda J, Hurtado C, Poniachik J, Brahm J Abstract Hepatitis B virus (HBV) related acute liver failure (ALF) is uncommon in our region, and there is limited HBV literature regarding the optimal management of these cases. In this article, we report two clinical cases of young men who have sex with men (MSM), both developed severe acute hepatitis caused by HBV, progressed to ALF and afterward required liver transplantation. Antiviral post-transplant treatment included entecavir without Hepatitis B Immunoglobulin (HBIG), and immunosuppression therapy with steroids, tacrolimus, ...
Publication date: Available online 19 October 2019Source: The Annals of Thoracic SurgeryAuthor(s): Leona T. Yarboro