Effect of H 2 O 2 induced oxidative stress (OS) on volatile organic compounds (VOCs) and intracellular metabolism in MCF-7 breast cancer cells
In this study, an OS model of MCF-7 cells was
constructed through H 2 O 2 -treatment, which for the first time combined volatile organic compounds
(VOCs) and intracellular metabolic analysis for a comprehensive and in-depth study of the OS effect
on cell metabolism. The VOCs produced by cells in H 2 O 2 -treated groups and control groups were
extracted with solid-phase microextraction (SPME) and analyzed by gas chromatography-mass
spectrometer (GC-MS). Meanwhile, the intracellular metabolites were extracted by methanol and
analyzed by GC-MS coupled with silyl-derivatization. The results indicated 15 VOCs and 29
intracellular metabolites were statistically different ( p
Source: Journal of Breath Research - Category: Respiratory Medicine Authors: Yuanling Liu, Wenwen Li and Yixiang Duan Source Type: research
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