Canada not doing enough to prevent disease from imported dogs

The Canadian Veterinary Medical Association says the federal government needs to update its rules for testing imported animals for communicable diseases, including brucellosis.
Source: CBC | Health - Category: Consumer Health News Tags: News/Health Source Type: news

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We report a new colorimetric immunoassay based on colored silica nanoparticles for detection of Brucella abortus. An immunosensor was designed based on blue-SiNPs and paramagnetic nanoparticles (PMNPs). The synthesized immunosensor was conjugated with a polyclonal antibody against B. abortus, which was activated by 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) and N-hydroxysuccinimide (NHS) to form detection and capture probes, respectively. After adding the conjugates to the bacterial suspension, sandwich structure of PMNPs B. abortus-blue-SiNPs was formed and then separated by a magnet. The blue dye was released f...
Source: Artificial Cells, Nanomedicine and Biotechnology - Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research
Publication date: Available online 19 June 2019Source: Comparative Immunology, Microbiology and Infectious DiseasesAuthor(s): Saber Esmaeili, Fahimeh Bagheri Amiri, Hamid Mokhayeri, Mohammad Hassan Kayedi, Max Maurin, Mahdi Rohani, Ehsan MostafaviAbstractMost zoonoses are occupational diseases. Q fever, brucellosis and tularemia are major zoonotic diseases for butchers and slaughterhouse workers. However, little information is available about these infectious diseases in such professional populations in western of Iran. The aim of this study was to investigate the seroprevalence and risk factors associated with these three...
Source: Comparative Immunology, Microbiology and Infectious Diseases - Category: Infectious Diseases Source Type: research
by Amahyel M. Gusi, Wilson J. Bertu, M. Jes ús de Miguel, Lucía Dieste-Pérez, Henk L. Smits, Reuben A. Ocholi, José M. Blasco, Ignacio Moriyón, Pilar M. Muñoz BackgroundBrucellosis is a world-wide extended zoonosis that causes a grave problem in developing economies. Animal vaccination and diagnosis are essential to control brucellosis, and the need for accurate but also simple and low-cost tests that can be implemented in low-infrastructure laboratories has been emphasized. MethodologyWe evaluated bovine, sheep, goat and swine lateral flow immunochromatography assay kits (LFA), th...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
The objective of  this study was to record the co-infection ofBrucella abortus andOnchocerca sp. as a first report of co-infection of these two agents in the same lesion. The sample constituted of a nuchal bursitis in the cervical ligament, a suggestive lesion common to these two agents, submitted to histopathology andBrucella spp. isolation in the Brucellosis reference laboratory of the Brazilian Ministry of Agriculture, Livestock and Food Supply. Brucellosis serological diagnosis were also performed in the animal ’s serum sample.B. abortus was isolated from the lesion and filarid nematode structures were ident...
Source: Journal of Parasitic Diseases - Category: Parasitology Source Type: research
This study aimed to evaluate the rate of Brucella spp. contamination of camel milk samples sent for analysis to the national brucellosis laboratory during 2018 in Iran. For this purpose, 96 milk samples from 96 dairy camel herds were randomly collected from two provinces and investigated for the presence of Brucella spp contaminations by both bacterial culture method and polymerase chain reaction (PCR). No clinical manifestation of brucellosis was reported in camels from which milk samples were collected. Using the culture method, three milk samples (3%) originating from two camels of Isfahan province (4%) and one camel fr...
Source: Preventive Veterinary Medicine - Category: Veterinary Research Source Type: research
AbstractBrucellosis in pregnant women is reported to be associated with obstetric complications (OCs), and adequate data for human brucellosis during pregnancy are largely lacking. We performed this multicenter retrospective cross-sectional study to evaluate the epidemiology, clinical course, treatment responses, and outcomes of brucellosis among pregnant women. The study period comprised a 14-year period from January 2002 to December 2015. All consecutive pregnant women diagnosed with brucellosis in 23 participating hospitals were included. Epidemiological, clinical, laboratory, therapeutic, and outcome data along with th...
Source: European Journal of Clinical Microbiology and Infectious Diseases - Category: Microbiology Source Type: research
ConclusionGesho leaf ethanol extract contains chemicals with anti-biofilm and bactericidal activities. This work lends support to the traditional use of gesho for treating topical infections and warrants further investigation into Rhamnus prinoides as a source of antibacterial and anti-biofilm agents.Graphical abstract
Source: Journal of Ethnopharmacology - Category: Drugs & Pharmacology Source Type: research
Roles of Dermcidin, Salusin-α, Salusin-β and TNF-α in the Pathogenesis of Human Brucellosis. Iran J Immunol. 2019 Jun;16(2):182-189 Authors: Sağmak Tartar A, Özer Balin Ş, Akbulut A, Yardim M, Aydin S Abstract BACKGROUND: Brucella spp. are facultative intracellular pathogens that can cause chronic infections in many tissues and organs. OBJECTIVES: To investigate serum dermcidin, salusin-alpha, salusin-beta and TNF-alpha levels and their correlation with each other in patients with acute brucellosis. METHODS: From 50 patients hospitalized upon diagnosis of acute brucellos...
Source: Iranian Journal of Immunology - Category: Allergy & Immunology Tags: Iran J Immunol Source Type: research
Determining the infectious cause of abortion in cattle is difficult. This case-control study was set up to investigate the infectious causes of abortion by determining the seroprevalence of three reproductive ...
Source: BMC Veterinary Research - Category: Veterinary Research Authors: Tags: Research article Source Type: research
This study aimed to evaluate the peripheral distributions of Th1 and Th17 cells and serum levels of IFN-γ, IL-17A and IL-22 cytokines in brucellosis patients. One hundred patients (36 acute, 41 under-treatment and 23 relapsed) and 30 age- and sex-matched healthy controls were included. The frequencies of Th1 and Th17 cells were determined by flow cytometric analysis. Serum levels of IFN-γ, IL-17A and IL-22 were measured by multi-analyte flow assay. Increased frequencies of Th1 and Th17 cells were observed in acute and relapsed brucellosis versus under-treatment patients and healthy controls (P
Source: Microbes and Infection - Category: Infectious Diseases Source Type: research
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