Demonstration of a quantitative triplex LAMP assay with an improved probe-based readout for the detection of MRSA

Analyst, 2019, Accepted Manuscript DOI: 10.1039/C9AN00671K, PaperImaly Nanayakkara, Ian Matthew White As molecular diagnostics move away from polymerase chain reaction (PCR) in order to target point-of-care testing applications, loop-mediated isothermal amplification (LAMP) is gaining popularity due its rapid, sensitive and specific... The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Analyst latest articles - Category: Chemistry Authors: Source Type: research

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ConclusionLME in combination with clotrimazole exhibited synergy effect against C. albicans and T. rubrum. In combination with artocarpin, it showed synergy effect against MRSA.
Source: Chinese Herbal Medicines - Category: Complementary Medicine Source Type: research
CONCLUSIONS: DM patients with poor glucose control are more susceptible to MRSA infection. They suffer from higher antimicrobial resistance, a higher co-infection rate, and much severer pneumonia than non-DM. MRSA itself is an independent risk factor for mortality in all patients. PMID: 31205100 [PubMed - in process]
Source: Chinese Medical Journal - Category: General Medicine Authors: Tags: Chin Med J (Engl) Source Type: research
Publication date: Available online 17 June 2019Source: Veterinary MicrobiologyAuthor(s): Igor Loncaric, Sarah Lepuschitz, Werner Ruppitsch, Alan Trstan, Thomas Andreadis, Nikolaos Bouchlis, Helene Marbach, Bernhard Schauer, Michael P. Szostak, Andrea T. Feßler, Frank Künzel, Theresia Licka, Burkhard Springer, Franz Allerberger, Stefan Monecke, Ralf Ehricht, Stefan Schwarz, Joachim SpergserAbstractThe aim of the present study was to investigate the diversity of methicillin-resistant Staphylococcus aureus (MRSA) that originated from Austrian companion animals during the last five-year period. A total of 90 non-rep...
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research
In this study, we examined the difference in bacterial colonization of different curtains. We experimentally contaminated antibacterial Fantex protective curtains and compared the bacterial counts to natural contamination of privacy curtains. There was a significant reduction in the CFU/cm2 on antibacterial Fantex protective privacy curtains after 24 hours of experimental contamination with Pseudomonas aeruginosa, Acinetobacter baumannii, methicillin-resistant Staphylococcus aureus (MRSA) or extended-spectrum-producing organisms (Escherichia coli or Klebsiella pneumoniae), compared to standard privacy curtains. Levels of e...
Source: Infezioni in Medicina - Category: Infectious Diseases Tags: Infez Med Source Type: research
In this study, we investigated the effects of short-chain fatty acids, metabolites produced by commensal bacteria, on the growth of S. aureus both in vitro and in vivo. Sodium propionate (NaP) most potently inhibited the growth of MRSA and multidrug-resistant clinical isolates. Of note, only NaP, but not NaA or NaB, ameliorated MRSA skin infection, significantly lowering bacterial load, excessive cytokine production, and the size and weight of abscesses approximately by two-fold. In addition, interestingly, S. aureus deficient of lipoteichoic acids (LTA) or wall teichoic acids (WTA), which are important in bacterial physio...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Two new diphenyl ethers, 1-[2-methoxy-4-(3-methoxy-5-methylphenoxy)-6-methylphenyl]-ethanone (1) and 1-[4-(3-(hydroxymethyl)-5-methoxyphenoxy)-2-methoxy-6-methylphenyl]-ethanone (2), together five known diphenyl ether derivatives (3 –7), were isolated from the fermentation products of an endophytic fungusPhomopsis fukushii. Their structures were elucidated by spectroscopic methods, including extensive 1D and 2D NMR techniques. Compounds1 and2 were evaluated for their anti-methicillin-resistantStaphylococcus aureus (anti-MRSA) activity. Compounds1 and2 showed good inhibition with IZD of 13.8 ± 1.5 mm and 14.6 &...
Source: Chemistry of Natural Compounds - Category: Chemistry Source Type: research
Two new isoflavones, 6-hydroxy-7-(3-hydroxypropyl)-4 ′-methoxyisoflavone (1) and 6,4 ′-dihydroxy-7-(3-hydroxypropyl)-isoflavone (2), were isolated from the flowers ofRosa damascena. Their structures were elucidated by spectroscopic methods, including extensive 1D and 2D NMR techniques. Compounds1 and2 were evaluated for their anti-methicillin-resistantStaphylococcus aureus (anti-MRSA) activity. The results revealed that compounds1 and2 showed weak inhibition with IZD of 11.6 ± 1.2 and 11.2 ± 1.0 mm, respectively. Compounds1 and2 were also tested for their antioxidant activity and showed good activ...
Source: Chemistry of Natural Compounds - Category: Chemistry Source Type: research
ConclusionA significant proportion of patients acquiredS. aureus during hospitalization. The finding of more than 90% ofS. aureus SSI to be of endogenous source underscores the need of improving infection prevention and control measures including screening and decolonization of high risk patients.
Source: Antimicrobial Resistance and Infection Control - Category: Infectious Diseases Source Type: research
Publication date: Available online 16 June 2019Source: MycoscienceAuthor(s): Hiromu Iwasaki, Toshiyuki Tokiwa, Mayu Shiina, Yukihiro Asami, Kazuro Shiomi, Satoshi Ōmura, Kenichi NonakaAbstractWe have already found some Metarhizium isolates produce euvesperins A and B, which were circumventors of arbekacin resistance in MRSA. These isolates were obtained from soil samples collected from the Izu islands and Kannami town, Sizuoka, Japan.Using a combination of micro-morphological characteristics and multigene (SSU, LSU, TEF) phylogenics, the isolates were indentified as a previously underscribed species of Metarhizium. Phylog...
Source: Mycoscience - Category: Biology Source Type: research
ConclusionsOur findings indicate that both MRSA decolonization strategies have an additional benefit in reducing the prevalence of MR-CoNS. Although the universal MRSA decolonization has superior efficacy in decolonization of CoNS, it may increase the risk of selecting CHX-R and Mu-R. In addition, other potential resistance genes should be studied.
Source: American Journal of Infection Control - Category: Infectious Diseases Source Type: research
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