Identification and characterization of 'readers' for novel histone modifications.

Identification and characterization of 'readers' for novel histone modifications. Curr Opin Chem Biol. 2019 May 10;51:57-65 Authors: Zhao S, Yue Y, Li Y, Li H Abstract Histone readers recognize histone modifications and mediate downstream biological events. A series of strategies to identify new histone readers have been developed and improved recently. Asides from the traditional pull-down methods and protein structure/function based educated guess, crosslinking and high-throughput screening based strategies led to the discovery of many new histone readers. In this review, we reviewed the rationale and applications of photo-affinity lysine based crosslinking strategies and array/designer nucleosome libraries based high-throughput screening strategies. Epigenome editing technologies to incorporate histone modifications in cells were also discussed. Finally, we summarized the newly identified histone readers (e.g. ZZ domain and Agenet domain) and histone modifications (e.g. serotonylation and benzoylation). PMID: 31082667 [PubMed - as supplied by publisher]
Source: Current Opinion in Chemical Biology - Category: Biochemistry Authors: Tags: Curr Opin Chem Biol Source Type: research