Nef-induced differential gene expression in primary CD4+ T cells following infection with HIV-1 isolates

In this study, we infected primary human CD4+ T cells with pseudotyped Nef-containing or Nef-deleted ( Δ-nef) NL4-3 virus and used RNA-Sequencing (RNA-Seq) for transcriptomic  analysis. Our results showed that the interferon response, IL-15 and JAK/STAT signaling, as well as genes involved in metabolism, apoptosis, cell cycle regulation, and ribosome biogenesis were all altered in the presence of Nef. These early Nef-mediated transcriptional alterations may play a role in priming the host cell for cellular activation and viral replication.

Source: Virus Genes - May 14, 2019 Category: Genetics & Stem Cells Source Type: research