Selenoprotein T as a new positive inotrope in the goldfish Carassius auratus [RESEARCH ARTICLE]

This study aimed to evaluate the cardiac expression of SELENOT, and the effects of exogenous PSELT (a 43-52 SELENOT derived-peptide) on the heart function of C. auratus, a hypoxia tolerance fish model. We found that SELENOT was expressed in cardiac extracts of juvenile and adult fish, located in the sarcoplasmic reticulum (SR) together with calsequestrin-2. Expression increased under acute hypoxia. On ex vivo isolated and perfused goldfish heart preparations, under normoxia, PSELT dose-dependently increased Stroke Volume (SV), Cardiac Output (Q), and Stroke Work (SW), by involving cAMP, PKA, L-type calcium channels, SERCA2a pumps, and pAkt. Under hypoxia, PSELT did not affect myocardial contractility. Only at higher concentrations (10–8 -10–7 M) an increase of SV and Q was observed. It also reduced the cardiac expression of 3-NT, a tissue marker of nitrosative stress which increases under low oxygen availability. These data are the first to propose SELENOT 43-52, PSELT, as a cardiac modulator in fish, with a potential protective role under hypoxia.

Source: Journal of Experimental Biology - May 12, 2019 Category: Biology Authors: Rosa, M., Alfonsina, G., Sandra, I., Loubna, B., Serena, L., Yamine, M. B., Mariacristina, F., Carmine, R., Tommaso, A., Youssef, A., Carmela, C. M. Tags: RESEARCH ARTICLE Source Type: research