Molecular detection of ALS1, ALS3, HWP1 and SAP4 genes in Candida Genus isolated from hospitalized patients in Intensive Care Unit, Tehran, Iran.

Molecular detection of ALS1, ALS3, HWP1 and SAP4 genes in Candida Genus isolated from hospitalized patients in Intensive Care Unit, Tehran, Iran. Cell Mol Biol (Noisy-le-grand). 2019 Apr 30;65(4):15-22 Authors: Ardehali SH, Azimi T, Fallah F, Aghamohammadi N, Alimehr S, Karimi AM, Azimi L Abstract Candida species are considered as one of the important cause of nosocomial and community infections. Candidacies are fourth caused by septicemia in some countries and possess extra cost to the health care system. The aim of this study was survey the presence of virulence factors associated with various candida geniuses in samples which have been collected from the intensive care unit. In this cross-sectional study, various clinical specimens have been collected from patients which hospitalized in the Intensive Care Unit (ICU) of Milad hospital, Tehran, Iran. The species of candida has been determined by CHROM agar. Finally, adherence factors genes and proteinase gene have been detected by PCR. In this research, 100 samples have been collected from patients that colonized with candida. C. albicans (63%) and C.glabrata (19.4%) are the most identified species, respectively. The species of four specimens have been not detected according to the color of CHROM agar candida medium and two different genus of candida has been isolated from 7 patients. The frequency of Als1, Als3, HWP1 and SAP1 genes among C. albicans was (92%), (94%), (95%) and (88%...
Source: Cellular and Molecular Biology - Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research