Three new triterpenoids transformed from ursolic acid by Mucor spinosus AS3.3450 and their cytotoxicity
Publication date: August 2019Source: Phytochemistry Letters, Volume 32Author(s): Shan-Shan Zhang, Ling-Shuang He, Yan-Min Zhao, Shan Fu, Dai-Lin Liu, Zi-He Yu, Bai-Chuan LiuAbstractMicrobial transformation of ursolic acid by Mucor spinosus AS 3.3450 was carried out in this paper. Three new compounds (1-3) were isolated and their structures were identified as 3β, 7β-dihydroxy-ursolic acid-28-ethanone (1), 3β, 7β, 21β-trihydroxy-ursolic acid (2), and 3β, 21β-dihydroxy-ursolic acid-3-O-β-D-glucopyranoside (3) based on extensive spectral analysis including HRESI-MS, 1H-NMR, 13C-NMR, HMQC and HMBC. Compared with the substrate, compound 1 had stronger cytotoxic activity against Hela cell, K562 cell and KB cell lines. Using Mucor spinosus AS 3.3450 to bio-transform ursolic acid for the first time, this study provided possible ways for reforming the chemical structure of UA and the obtained activity-enhanced bio-transformation products may lay a foundation for the development and application of lead compounds in the future.Graphical abstract
Source: Phytochemistry Letters - Category: Chemistry Source Type: research