Cytotoxicity, antioxidant, anti-inflammatory activity, and GC-MS analysis of Egyptian propolis

AbstractNatural products have a great contribution to folk medicine because it is rich in various and unique compounds. Propolis is considered one of the most important natural products due to their different medical applications. In this work, the antioxidant, anti-inflammatory, and cytotoxicity of propolis to cancer cell lines were investigated which was obtained from Bee Research Department, Plant Protection Research Institute and Agricultural Research Center, Egypt. The antioxidant activity was evaluated by DPPH assay. The propolis concentration of 1280  μg/ml achieved the highest value (91.42%) of the antioxidant activity in ethanolic extract of propolis. Moreover, the cytotoxicity of propolis was evaluated using MTT assay to two cell lines, A-549 and cervical carcinoma cells, with IC50 of approximately 26.4  ± 3.6 μg/ml and 117 ± 1.2 μg/ml. The propolis concentration of 1000 μg/ml achieved the highest value (71.14%) of anti-inflammatory activity in the ethanolic extract of propolis while the concentration of 7.81 μg/ml achieved the lower potential (12.93) of anti-inflammatory activity with IC50 = 68.52 μg/ml. The phytochemical components and characteristics of the ethanol extract of propolis were determined by using GC-MS analysis. Our results emphasized that the propolis has biologically active compounds.
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research

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ConclusionSCCA, FBG, and a combination of SCCA and FBG could acceptably predict CR following NACT. Pretreatment SCCA and FBG levels were independent prognostic factors. The combination of SCCA and FBG levels refined the prognostic stratification of LACC patients, which allowed the group of patients with the highest risk of recurrence and death to be identified.
Source: Cancer Medicine - Category: Cancer & Oncology Authors: Tags: ORIGINAL RESEARCH Source Type: research
CONCLUSIONS: Monalizumab 10 mg/kg IV q 2 weekly is well tolerated in patients with pre-treated gynecologic cancers. Short term disease stabilization was observed. Future studies should assess combinations with other agents including immunotherapeutics. PMID: 31308062 [PubMed - as supplied by publisher]
Source: Clinical Cancer Research - Category: Cancer & Oncology Authors: Tags: Clin Cancer Res Source Type: research
ConclusionNACT with taxane/platin followed by definitive CT/RT is as effective as the standard care in the treatment of locally advanced cervical cancer. It has even shown better results (p value  >  .005) and is also helpful in reducing systemic micrometastasis and bulk of the disease. It can be used as an alternative to the standard care at the places of long waiting time for the definitive treatment, without compromising the outcome.
Source: The Journal of Obstetrics and Gynecology of India - Category: OBGYN Source Type: research
This article is accompanied by a video that provides step-by-step demonstration of a uterine artery–sparing robotic-assisted radical trachelectomy in a patient with stage IB1 squamous cell carcinoma of the cervix, a 2-cm mass, and a desire for future fertility. We also conducted a review of the literature examining the use of uterine artery–sparing techniques among minimally invasive radical trachelectomies. Using PubMed, Google Scholar, and Ovid search tools, 28 total publications were reviewed, of which 16 were eligible for use in our comparison. With the addition of our case report, a total of 154 cases spar...
Source: Journal of Minimally Invasive Gynecology - Category: OBGYN Source Type: research
CONCLUSIONS: One in eight transplant recipients developed lower genital tract dysplasia and approximately half were high-grade dysplasia or cancer. Black race and hydroxychloroquine use were associated with an increased risk of dysplasia. Yearly cervical screening and comprehensive lower genital examination beyond the cervix is indicated in this population. PMID: 31306332 [PubMed - as supplied by publisher]
Source: Obstetrics and Gynecology - Category: OBGYN Authors: Tags: Obstet Gynecol Source Type: research
CONCLUSION: We found that the prognosis of OV is not poor in our series. Stage (IV) and tumor size are the main risk factors in metastatic development. PMID: 31273353 [PubMed - as supplied by publisher]
Source: Hellenic Journal of Nuclear Medicine - Category: Nuclear Medicine Tags: Hell J Nucl Med Source Type: research
CONCLUSION: An increased SUVmax suggests lymph node metastases or distant metastases, low differentiation and FIGO stage III-IV. A low negative correlation was observed between the SUVmax and ADCmin, while we observed no correlation between the ADCmin and the clinical pathological characteristics of cervical cancer. PMID: 31273350 [PubMed - as supplied by publisher]
Source: Hellenic Journal of Nuclear Medicine - Category: Nuclear Medicine Tags: Hell J Nucl Med Source Type: research
Authors: Liu Z, Shi H Abstract Objective: To systematically evaluate the significance of squamous cell carcinoma antigen (SCC-Ag) in the prognosis of cervical cancer. Methods: Literature from Pubmed, Embase, and Cochrane Library was retrieved to collect all English literature on the correlation between SCC-Ag and cervical cancer prognosis, and the quality of literature collected was assessed based on evaluation criteria. The heterogeneity, sensitivity, and specificity were detected using the StataSE12.0 software, and the correlation between SCC-Ag and cervical cancer prognosis as the effect variables was assess...
Source: Disease Markers - Category: Laboratory Medicine Tags: Dis Markers Source Type: research
Cervical cancer is the second most prevalent cancer worldwide. Portulaca oleracea L. polysaccharide (POL-P3b) has been found to have enhancing immune and anti-cervical cancer activity by oral administration. Dend...
Source: BMC Complementary and Alternative Medicine - Category: Complementary Medicine Authors: Tags: Research article Source Type: research
In this study, quantitative real-time PCR (qRT-PCR) was used to detect miR-145 and FSCN1 expression levels in tissues and HeLa cells. Western blotting was performed to determine the protein level of FSCN1. The luciferase assay was used to verify the direct target of miR-145. The CCK-8 assay and 2D colony formation assays were performed to determine the effects of miR-145 mimics or FSCN1 silencing on cell proliferation. miR-145 expression levels were significantly down-regulated, while FSCN1 expression levels were significantly up-regulated in the cervical carcinoma tissues compared with their matched non-cancerous tissues....
Source: Cell Transplantation - Category: Cytology Authors: Tags: Cell Transplant Source Type: research
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