Application of SPME supported by ionic liquids for the determination of biogenic amines by MEKC in clinical practice

Publication date: Available online 11 May 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Natalia Kossakowska, Ilona Olędzka, Alicja Kowalik, Natalia Miękus, Piotr Kowalski, Alina Plenis, Ewa Bień, Aleksandra Kaczorowska, Małgorzata Anna Krawczyk, Elżbieta Adamkiewicz-Drożyńska, Tomasz BączekAbstractThe analysis of biogenic amines (BAs) and their metabolites is helpful for the diagnosis of central nervous system disorders and other neuroendocrine and cancer disturbances. In the study, a developed micellar electrokinetic chromatography method, coupled with diode array detection (MEKC-DAD), was validated to monitor levels of adrenaline (A), noradrenaline (NA), dopamine (DA), L-Tryptophan (L-Tryp) and L-Tyrosine (L-Tyr) in real human urine samples. These neurotransmitters were isolated from urine samples using solid-phase microextraction (SPME) and methanol containing 1-ethyl-3-methylimidazolium tetrafluoroborate ionic liquid as the desorption phase. The method was linear for DA, A and L-Tyr in the range of 0.5-20 µg/mL and for NA and L-Tryp in the range of 0.25-20 µg/mL. The good linearity for BAs was confirmed by the correlation coefficient (R2) from 0.9989 for A to 0.9997 for NA and L-Tryp, respectively. The validation assays for accuracy, precision, limit of detection, limit of quantification, absolute recovery, and stability of the analytes were consistent with the requirements recommended by the FDA and ICH guidelines. Next, the valida...
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research