Binding propterties of two Ru(II) polypyridyl complexes containing dppz units and fluorine groups with poly(U) ·poly(A) ∗ poly(U) triplex.

Binding propterties of two Ru(II) polypyridyl complexes containing dppz units and fluorine groups with poly(U)·poly(A) ∗ poly(U) triplex.
J Inorg Biochem. 2019 May 02;197:110705
Authors: Wang F, Ma S, Feng Y, Liu X, Tan L
Abstract
In this work, two Ru(II)-dppz (dppz = dipyrido[3,2-a:2',3'-c]phenazine) complexes containing fluorine substituents, [Ru(bpy)2(7-F-dppz)]2+ (Ru1, bpy = 2,2'-bipyridine, 7-F-dppz = 7-fluorodipyrido[3,2-a:2',3'-c]phenazine) and [Ru(phen)2(7-F-dppz)]2+ (Ru2, phen = 1,10-phenanthroline), have been synthesized and characterized. Binding properties of Ru1 and Ru2 with the RNA poly(U)·poly(A) ∗ poly(U) triplex have been studied by spectroscopic methods and viscosity measurements. The obtained results indicate that the binding differences of the two complexes with the triplex may be attributed to the ancillary ligand effects, implying that the better planarity and greater hydrophobicity of ancillary ligands are advantageous to the π-π stacking interaction between Ru2 and the triplex, thus Ru2 stabilizes the triplex strongly than Ru1. Denaturation of the triplex shows that both Ru1 and Ru2 can not only highly stabilize the template duplex of the triplex, but also significantly stabilize the third strand. Compared with the triplex stabilizing effects for the reported Ru(II)-dppz complexes, thermal melting experiments suggest that the fluorine substituent on the ligand dppz can probably ...

Source: Journal of Inorganic Biochemistry - May 1, 2019 Category: Biochemistry Authors: Wang F, Ma S, Feng Y, Liu X, Tan L Tags: J Inorg Biochem Source Type: research