Strain-dependent effects on lung structure, matrix remodeling and Stat3/Smad2 signaling in C57BL/6N and C57BL/6J mice after neonatal hyperoxia.

Strain-dependent effects on lung structure, matrix remodeling and Stat3/Smad2 signaling in C57BL/6N and C57BL/6J mice after neonatal hyperoxia. Am J Physiol Regul Integr Comp Physiol. 2019 May 08;: Authors: Will JP, Hirani DV, Thielen F, Klein F, Vohlen C, Dinger K, Dötsch J, Alejandre-Alcázar MA Abstract Bronchopulmonary dysplasia (BPD) is a chronic lung disease of preterm infants, characterized by lung growth arrest and matrix remodeling. Various animal models provide mechanistic insights in the pathogenesis of BPD. Since there is increasing evidence that genetic susceptibility modifies the response to lung injury, we investigated strain-dependent effects in hyperoxia-induced lung injury of newborn mice. To this end, we exposed newborn C57BL/6N and C57BL/6J mice to 85% O2 (HYX) or to 21% O2 (NOX) for 28 days, followed by lung excision for histological and molecular measurements. (1) BL/6J-NOX exhibited a lower body and lung weight than BL/6N-NOX; hyperoxia reduced body weight in both strains, and increased lung weight only in BL/6J-HYX. (2) Quantitative histomorphometric analyses revealed reduced alveolar formation in lungs of both strains after hyperoxia, but the effect was greater in BL/6J-HYX than BL/6N-HYX. Septal thickness was lower in BL/6J-NOX than BL/6N-NOX, but increased in both strains after hyperoxia. Elastic fiber density was significantly greater in BL/6J-HYX than BL/6N-HYX. (3) Lungs of BL/6J-HYX were protected from...
Source: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology - Category: Physiology Authors: Tags: Am J Physiol Regul Integr Comp Physiol Source Type: research