The Effect of Wnt Pathway Modulators on Human iPSC-Derived Pancreatic Beta Cell Maturation

In this study, we stimulated hiPSC-derived S7 cells with canonical and non-canonical Wnt signaling pathways with synthetic proteins including WNT3A, WNT4, WNT5A and WNT5B as well as inhibition of endogenous Wnt signaling with the Tankyrase inhibitor G007-LK (TKi). Our data show that neither canonical nor non-canonical Wnt stimulation alone is enough to mature β-cells, however, global proteomics profiling of TKi-treated S7 cells showed more similarity to adult human islets, suggesting that inhibition of endogenous Wnt contribute towards final β-cell maturation.
Source: Frontiers in Endocrinology - Category: Endocrinology Source Type: research