Rapid identification and quantification of Lactobacillus rhamnosus-targeting real-time PCR using a TaqMan probe.

In this study, we developed an L. rhamnosus-targeting quantitative PCR assay, which produces accurate and reproducible results based on the specificity of a TaqMan probe targeting the unique 16S rDNA sequence of L. rhamnosus. The assay specifically detected the targeted bacterium, L. rhamnosus, and no non-specific signals were generated under the study conditions. Using genomic DNA from the bacterial cells of L. rhamnosus (101 to 106 cells), the cycle threshold value showed a linear trend (R2 = 0.9993). This L. rhamnosus-targeting quantitative PCR assay can contribute to advance research into the effects of the organism on microflora, microbial infections, and the host. PMID: 31061362 [PubMed - as supplied by publisher]
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Jpn J Infect Dis Source Type: research