Comparative study on hemato-biochemical alterations and selected acute phase protein response in native sheep experimentally infected with bluetongue virus serotypes 10 and 24

AbstractBluetongue (BT) is an infectious, non-contagious, insect-transmitted viral disease of sheep, other domestic, and wild ruminants caused by bluetongue virus (BTV), a prototype species of the genusOrbivirus of the familyReoviridae. The study was designed to determine the hematological and biochemical alterations and acute phase protein response in BTV sero-negative sheep experimentally infected with bluetongue virus serotype-10 and 24. The BTV-infected group comprising of 18 animals, 6 each inoculated with 6  ml of clarified virus containing 1 × 106/ml TCID50 of BTV-10 or 24 by intradermal route, 6 animals inoculated with equal volumes of BTV-10 and 24 and 4 animals with 6  ml of mock infected culture fluid served as uninfected control group. The blood and serum samples were analyzed at 0, 1, 3, 7, 11, and 16 days post-infection (DPI). Hematological findings in this study showed a significant decrease in the total number of white blood cells in BTV-10 infected group , a significant increase in circulating neutrophils decrease in lymphocytes in all the infected groups, in PCV in BTV-24 and co-infected groups, and RBC count in only BTV-24-infected group at 11 DPI. The results of serum biochemical analyses of alanine aminotransferase (ALT), aspartate aminotransfer ase (AST), and alkaline phosphatase (ALP) exhibited increased levels in BTV-24 and co-infected animals. A significant rise in the serum level of creatine kinase (CK) was observed from 3 DPI in the infected...

Source: Comparative Clinical Pathology - May 7, 2019 Category: Pathology Source Type: research