The effect of Descurainia sophia oil on methamphetamine-induced cell cytotoxicity and cell death in PC12

In this study, protective effects ofDescurainia sophia oil were followed up in methamphetamine-induced cell cytotoxicity in a neuron-like PC12 cell line. The viability, proliferation, and cytotoxicity of the cells were assessed by Trypan blue, MTT test, and lactate dehydrogenase (LDH) assay, respectively. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) test was performed to detect DNA fragmentation and apoptosis. Nitric oxide (NO) level was detected by Griese method. Interleukin-1 β (IL-1β), IL-6, interferon gamma (INF-γ), and tumor necrosis factor alpha (TNF-α) pro-inflammatory cytokines were measured by Rat Kit V-Plex, and finally, caspase-3 activity was determined by spectrophotometry. Results showed thatDescurainia sophia oil has cell death –suppressing effects on PC12 cells. It enhanced the cell viability and proliferation and also increased the cell cytotoxicity and cell death index in methamphetamine-treated PC12 cells. Also, it suppressed NO production, inflammatory cytokine production by flow cytometry, mitochondrial membrane de polarization, and caspase-3 activity in a dose-dependent manner. We concluded thatDescurainia sophia oil suppresses the methamphetamine-induced cell death in PC12 cell due to reduction of NO production, inflammation, and inhibition of apoptosis cascade.
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research

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The original version of this article unfortunately contained a mistake in the acknowledgement section.
Source: Journal of Neurology - Category: Neurology Source Type: research
Source: Journal of Neurology - Category: Neurology Source Type: research
Source: Journal of Neurology - Category: Neurology Source Type: research
Source: Journal of Neurology - Category: Neurology Source Type: research
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Source: Journal of Neurology - Category: Neurology Source Type: research
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Source: Journal of Neurology - Category: Neurology Source Type: research
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Source: Journal of Neurology - Category: Neurology Source Type: research
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Source: Journal of Neurology - Category: Neurology Source Type: research
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Source: Journal of Neurology - Category: Neurology Source Type: research
In conclusion, cerebral hemispheric infarction limited to the PIVC or TPSVC does not necessarily cause vertigo. However, unilateral hemispheric infarctions, restricted to the areas belonging to the vestibular cortical network may cause vestibul ar symptoms. The lesions responsible for vestibular symptoms are located more often in the right hemisphere.
Source: Journal of Neurology - Category: Neurology Source Type: research
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