Immunoregulatory properties of cell-free DNA in plasma of celiac disease patients - A pilot study.

Immunoregulatory properties of cell-free DNA in plasma of celiac disease patients - A pilot study. Autoimmunity. 2019 May 06;:1-7 Authors: Brynychova I, Zinkova A, Hoffmanova I, Korabecna M, Dankova P Abstract The elevated plasma cell-free DNA (cfDNA) concentrations were repeatedly reported in association with the process of inflammation. The qualitative and quantitative characteristics of plasma cfDNA in active (newly diagnosed) celiac disease patients (CD) have not yet been studied despite the fact that cfDNA of healthy individuals is able to regulate immune response. We determined the total cfDNA concentration and relative content of telomeric sequences in plasma cfDNA in CD (n = 10) and healthy age- and sex-matched controls (HC, n = 10) by quantitative PCR. To obtain the evidence that the observed biological effects are caused solely by cfDNA molecules, we applied the treatment of paired plasma samples with DNase. Using paired samples of plasma (non-treated/native and treated by DNase), we analyzed the contribution of cfDNA to the activation of TLR9 and TNF-α mRNA expression in THP1 monocytic cell line. There were no significant differences in the quantities of plasma cfDNA and relative contents of telomeric sequences in their pools. When we compared the levels of TNF-α mRNA expression in THP1 cells achieved after stimulation with native CD and HC plasma samples, we found significantly (p = .031) higher expression a...
Source: Autoimmunity - Category: Allergy & Immunology Tags: Autoimmunity Source Type: research