NMR Studies of the Interaction of Calmodulin with IQ Motif Peptides

We describe here methods for the robust expression and purification of CaM isotopically enriched for NMR analysis, as well as for the complex of CaM with a peptide derived from the IQ motif sequence of the human cardiac sodium channel NaV1.5. We also describe methods for NMR analysis of titrations of CaM with IQ motif peptides to determine the stoichiometry of the complex and to identify the residues at the binding interface.
Source: Springer protocols feed by Protein Science - Category: Biochemistry Source Type: news