Salt-containing aqueous two-phase system shows predictable partition of proteins with surface amino acids residues.

In this study, we demonstrate that the partition of proteins in salt-containing ATPS (SATPS) depends only on the relation between the protein-surface amino acids and the type of salt using SATPS. The partition coefficients of four proteins changed depending on the kind of salt, according to the Hofmeister series. Interestingly, the partition coefficients of the proteins correlated to those of the combination of the amino acids in the surface of the protein with the correlation coefficients of >0.9. The results suggest that the interaction between the protein surface and aqueous ions plays an indispensable role for the partition of proteins in SATPS that can help in the design of protein partition in ATPS for purification and enrichment. PMID: 31055113 [PubMed - as supplied by publisher]
Source: International Journal of Biological Macromolecules - Category: Biochemistry Authors: Tags: Int J Biol Macromol Source Type: research