Urine as a promising sample for Leishmania DNA extraction in the diagnosis of visceral leishmaniasis - a review.

Urine as a promising sample for Leishmania DNA extraction in the diagnosis of visceral leishmaniasis - a review. Braz J Infect Dis. 2019 May 01;: Authors: Bezerra GSN, Barbosa WL, Silva EDD, Leal NC, Medeiros ZM Abstract Visceral leishmaniasis (VL) is a serious and debilitating infection with high fatality rate in tropical and subtropical countries. As clinical symptoms of VL are not so specific, confirmatory diagnostic methods with high sensitivity and specificity are needed. Noninvasive methods have been developed using urine as a clinical sample for VL diagnosis. In fact, there is a clear correlation between kidney impairment and Leishmania DNA in urine. However, it has been proved that Leishmania nucleic acid may also be isolated from patients without any sign of renal involvement. Even though urine has become a promissing biological sample, it is still not widely used due to several issues, such as (i) incomprehension of the whole renal pathophysiology process in VL, (ii) presence of many amplification inhibitors in urine, and (iii) lack of an efficient urinary DNA extraction method. In this article, we performed a literature review to bring a new perspective for Leishmania DNA isolation in urine. PMID: 31054271 [PubMed - as supplied by publisher]
Source: Braz J Infect Dis - Category: Infectious Diseases Authors: Tags: Braz J Infect Dis Source Type: research