Soy isoflavones (from Glycine max) preserves hepatocellular free radical defense system potential and ameliorates inflammatory response in high fat fed ovariectomised Wistar rats: The molecular mechanisms

Publication date: Available online 4 May 2019Source: Nutrition, Metabolism and Cardiovascular DiseasesAuthor(s): P. Sankar, P. Rajaa Muthu, Zachariah Bobby, M.G. SridharAbstractPostmenopausal women are more prone to develop metabolic syndrome (MetS) components; insulin resistance, type2 diabetes, dyslipidemia, atherosclerosis and obesity than premenopausal women. Oxidative stress (OxS) and inflammation (INF) are emerged as major triggers for these metabolic consequences. However, the pathogenesis of OxS and inflammation after menopause is only partially understood which is yet to be elucidated. The lack of safe/effective conventional treatments for postmenopausal women has changed the focus to natural products as the alternative remedy. We investigated the protective effects of soy isoflavones (SIF) on the pathogenesis of OxS and INF in an experimental model of postmenopausal obesity. Soy isoflavones were extracted and analyzed by HPLC method. In animal experiments, the sham operated and ovariectomised (OVX) Wistar rats were fed with either normal chow or high fat diet (HFD) in the presence and absence of soy isoflavones (150mg/kg bw/day/rat) for eight weeks. The blood and tissue OxS and INF signaling parameters were studied using appropriate biochemical and molecular techniques. Proteomics studies revealed that, OVX and HFD per se caused OxS and INF. This was attributed by suppressed expression of Nrf2, HO-1 and catalase (CAT) in association with enhanced expression of MPO a...
Source: Nutrition, Metabolism and Cardiovascular Diseases - Category: Nutrition Source Type: research