New chemical products formation from textile dye degradation, chitinolytic and antioxidant activity in new strain nbpc5-18 of Cellulosimicrobium sp. TH-20

Publication date: Available online 2 May 2019Source: Journal of Environmental Chemical EngineeringAuthor(s): Bilquees Tabasum, Prajakta R. Dhagale, Kirti M. Nitnaware, Harichandra A. Nikule, T.D. NikamAbstractThe new strain nbpc5-18 of Cellulosimicrobium TH-20, isolated from the filaments of Scytonema sp., was characterized by phenotypic, chemotaxonomic and 16S rRNA gene sequencing. Nucleotide homology and phylogenetic analysis indicated the affiliation of bacterium strain nbpc 5-18 (accession No. MH298840) with Cellulosimicrobium TH-20 but chemotaxonomic features differed from the related taxa. G + C content of test isolate was found to be 57%. Scanning electron microscopy showed rod-circular shaped isolate with length and width of 790 nm and 469.8 nm. The strain showed maximum growth in nutrient broth medium, at 35 ± 2ᵒ C and pH at 6.0–8.0. The strain was efficiently able to decolorize a wide range of laboratory (67-98%) and textile (74-94%) dyes. Structures of the products resulting from the bacterial degradation of dyes were identified by GC-MS and HR-MS. Mass spectra revealed the presence of some new low molecular weight compounds in the degradation products of dyes (Methylene blue, Bromocresol purple, Methyl orange, Rhodamine 6 G, Coomasie brilliant blue R-250 and Crystal violet). Azoreductase, laccase, lignin peroxidase, tyrosinase, and NADH-DCIP reductase activity were found to be higher in dye treated samples as compared to control signifying cruci...
Source: Journal of Environmental Chemical Engineering - Category: Chemistry Source Type: research