Contrast-induced nephropathy in an animal model: Evaluation of novel biomarkers in blood and tissue samples

Publication date: Available online 2 May 2019Source: Toxicology ReportsAuthor(s): Charalampos Mamoulakis, Irene Fragkiadoulaki, Phaedra Karkala, Georgios Georgiadis, Ioannis-Erineos Zisis, Polychronis Stivaktakis, Alexandra Kalogeraki, Ioannis Tsiaoussis, Tatyana Burykina, George Lazopoulos, Konstantinos Tsarouhas, Dimitrios Kouretas, Aristides TsatsakisAbstractIdentification of novel biomarkers of contrast-induced nephropathy (CIN) that may more accurately detect renal function changes; reflect kidney damage; assist monitoring; and elucidate pathophysiology attract considerable scientific attention nowadays. To evaluate novel biomarkers of nephrotoxicity in blood/tissue samples of a CIN model, 10 New Zealand white rabbits were divided into group 1 (n = 5; iopromide) and group 2 (n = 5; control). Blood was drawn at 0 h (immediately), 24 h and 48 h after contrast medium (CM) administration. Animals were euthanized at 48 h and kidneys were removed. Serum creatinine (sCr)/symmetric-asymmetric dimethylarginine (SDMA-ADMA) levels were measured. CM genotoxic/cytotoxic effect was investigated 48 h post-CM exposure using micronucleus assay in lymphocytes. Cytological examination was conducted using touch preparation technique (TPT). All animals in group 1 developed CIN: mean sCr levels increased by 68.2% within 48 h. Significant SDMA-ADMA level elevation was observed at 0 h and 24 h with insignificant drop at 48 h in group 1, remaining normal in group 2 at all time-points. Significan...
Source: Toxicology Reports - Category: Toxicology Source Type: research

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