Dual RNA-Seq Unveils Pseudomonas plecoglossicida htpG Gene Functions During Host-Pathogen Interactions With Epinephelus coioides

Conclusion This is the first report of the successful use of tissue dual RNA-seq to simultaneously monitor the dynamics of gene expression changes of pathogen and host in the context of prokaryotic and eukaryotic biological systems, which obtained high-resolution transcriptome data. Dual RNA-seq not only increased our knowledge of HtpG control of virulence gene expression in P. plecoglossicida during infection but also revealed the htpG-independent and htpG-dependent defense mechanisms of E. coioides against P. plecoglossicida. Furthermore, dual RNA-seq analysis also offered new clues underlying the pathological cause of granulomas. Notably, HtpG acts as a chaperone that enables the optimal folding of newly synthesized proteins under stress conditions, especially the change of temperature, while the virulence of P. plecoglossicida is temperature-dependent. Therefore, HtpG might act as a temperature sensor and effector, by regulating the expression of virulence genes, and then initiate the infection process at the pathogenic temperature. The ability of HtpG to respond to the environment is also likely to be activated by the adverse environment inside the host after the pathogen invasion, and then regulate the expression of virulence genes and interact with the host. In the future, we intend to validate these results and explain the molecular factors that govern these interactions during infection. Author Contributions QY and YS conceived the experiments. LH, LZ, WL, XX, and...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research