Plausible role of bacterial toxin –antitoxin system in persister cell formation and elimination
Targeting toxin ‐antitoxin systems for elimination of persisters:Under the influence of several micro‐environmental factors (temperature, pH etc…), the antitoxin component gets degraded resulting in a proportionate increase of toxin levels (T> A) that induces the formation of persister bacteria (red colour). On three varying conditions (Conditional cooperativity, Cellular detoxification and Use of toxin inhibitors), the antitoxin level becomes high (A> T) resulting in awakening of persister bacteria. After regrowth of persisters (green colour), antimicrobials can be applied to eliminate these pathogens. Although, a large proportion of pathogenic bacteria gets eliminated from hosts after antibiotic treatment, a fraction of population confronts against such effects and undergoes growth arrest to form persisters. Persistence in bacteria is a dormant physiological state where cells escape the effects of antimicrobials as well as other host immune defences without any genetic mutations. The state of dormancy is achieved through various complex phenomena and it is known that a gene pair named as toxin –antitoxin (TA) acts as a key player of persister cell formation where the toxin is activated either stochastically or after an environmental insult, thereby silencing the physiological processes. However, the controversial role of TA modules in persister cell formation has also been documented wi th reasonable clarity. Persisters may revert back from state of q...
Authors: Karimi G, Vard B, Riyahi R, Motlagh ME, Heshmat R, Kelishadi R Abstract This cross-sectional study was conducted among 14,400 Iranian students, aged 7-18 years to determine the association between the frequency of family dinner and mental health. Family dinner frequency, family relationship, life satisfaction (LS), self-perceived health and anxiety were assessed by validated questionnaires. Of 14,274 participants, 50.6% were boys and 71.4% were urban residents, with a mean (SD) age of 12.28 (3.16) years. Family dinner frequency was significantly correlated with LS (boys: β = 0.27, p
In this study we explored the use of a natural compound rubusoside (RUB) as a solubilizer to enhance the solubility of a fluorescence-labeled C6-Ceramide (NBD C6-Ceramide) and to characterize its pharmacokinetics and tissue distribution in an animal model. RUB significantly enhanced the solubility of NBD C6-Ceramide by forming nanomicelles, and efficiently delivered NBD C6-Ceramide in rats by oral and intravenous administration. RUB loaded 1.96% of NBD C6-Ceramide in the nanomicelles and solubilized it to a concentration of 3.6 mg/mL in water. NBD C6-Ceramide in nanomicelles remained stable in aqueous solutions, all...
Publication date: Available online 10 December 2019Source: Prostaglandins &Other Lipid MediatorsAuthor(s): Giorgio Mottola, Evan Werlin, Bian Wu, Mian Chen, Anuran Chatterjee, Melinda Schaller, Michael S. ConteAbstractInflammation ensuing from vascular injury promotes intimal hyperplasia (IH) and restenosis. Resolvin D1 (RvD1) is a lipid mediator that attenuates IH in vivo when delivered locally to the vessel wall in animal models. We tested the hypothesis that peri-procedural oral administration of RvD1 could blunt the local inflammatory response to angioplasty, and attenuate downstream IH. Carotid angioplasty was per...
First-of-its-kind trial evaluates decision aid’s efficacy
Authors: Glauser W PMID: 31818934 [PubMed - in process]
Authors: Owens B PMID: 31818933 [PubMed - in process]
Authors: Dermer M, Martel J, Greenhill A PMID: 31818932 [PubMed - in process]
Authors: Robson R PMID: 31818931 [PubMed - in process]
Authors: Lamb S PMID: 31818930 [PubMed - in process]
Authors: Zimmer V, Glanemann M, Lammert F PMID: 31818929 [PubMed - in process]