Transport and Recovery of Gilthead Sea Bream (Sparus aurata L.) Sedated With Clove Oil and MS222: Effects on Oxidative Stress Status

In conclusion, our results indicate that the use of both anesthetic agents, CO and MS222, interfere with fish antioxidant status. All tested biological matrices displayed alterations in antioxidant endpoints, confirming that these substances, although minimizing the effects of transport stress, may have long term effects on fish defenses. These results are of high relevance to aquaculture considering that an altered oxidative stress, induced by use of these anesthetics as stress-reducing agent during transport, may increase the susceptibility to different environmental or biotic stress and promote different types of pathologies. Ethics Statement The experiment complied with the Guiding Principles for Biomedical Research Involving Animals (EU2010/63), the guidelines of the Spanish laws (law 32/2007 and RD 53/2013), and authorized by the Ethical Committee of the Universidad de Cádiz (Spain) for the use of laboratory animals and the Ethical Committee from the Andalusian Government (Junta de Andalucía reference number 28-04-15-241). Author Contributions JM and IJ-C designed the experiments and performed the experimental setup. IJ-C, MT, and MO conducted the experimental procedures. MT, MO, LF-M, and AT did the laboratorial analysis and analyzed the results. MT and MO wrote the manuscript. LT and JM revised the manuscript. All authors read and approved the manuscript for publication. Funding This work was supported by the “Plan Nacional de Investigaci&#x...
Source: Frontiers in Physiology - Category: Physiology Source Type: research