Generation of temperature sensitive mutations with error-prone PCR in agene encoding acomponent of the spindle pole body in fission yeast.

We report here asimple procedure to generate ts mutations using error-prone PCR within pcp1 that encodes aspindle pole body (SPB) component in Schizosaccharomyces pombe. This manipulation is not restricted to pcp1, and can be suited to any essential genes involved in other processes.
PMID: 31042107 [PubMed - as supplied by publisher]

Source: Bioscience, Biotechnology, and Biochemistry - April 30, 2019 Category: Biochemistry Authors: Tang NH, Fong CS, Masuda H, Jourdain I, Yukawa M, Toda T Tags: Biosci Biotechnol Biochem Source Type: research