Vaccination with FAdV-8a induces protection against inclusion body hepatitis caused by homologous and heterologous strains.

Vaccination with FAdV-8a induces protection against inclusion body hepatitis caused by homologous and heterologous strains. Avian Pathol. 2019 May 01;:1-30 Authors: Steer-Cope PA, Sandy JR, O'Rourke D, Scott PC, Browning GF, Noormohammadi AH Abstract Fowl aviadenoviruses (FAdV) are an important avian pathogen, responsible for several poultry diseases prevalent worldwide, including inclusion body hepatitis (IBH). FAdV intraspecies cross-protection has been clearly demonstrated, but there is little evidence that any interspecies cross-protection exists. The present study aimed to assess the inter- and intraspecies protection between three FAdV field isolates (FAdV-8a, FAdV-8b, FAdV-11) identified in association with severe IBH outbreaks. Inoculums prepared using inactivated plaque-purified virus with adjuvant Montanide™ ISA 71VG, were injected intramuscularly into three-week-old SPF chickens. At six-weeks of age the birds were challenged with 106TCID50 of homologous or heterologous virus intraperitoneally, and full post-mortem examination performed at day four post-challenge. Various tissues were examined for gross and histological lesions and assessed for presence of virus by PCR-HRM. All homologous-type vaccine/challenge groups exhibited protection against IBH lesions with no virus detected in the tissues. Unvaccinated groups challenged with virus showed evidence of FAdV-induced lesions, however FAdV-8a demonstrated lower pathogenicity compared wit...
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research

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Source: Journal of Comparative Pathology - Category: Pathology Source Type: research
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Source: Transboundary and Emerging Diseases - Category: Veterinary Research Authors: Source Type: research
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Abstract During the period from 2015 to 2017, frequent outbreaks of inclusion body hepatitis (IBH) were observed in chicken broilers and falcons in Saudi Arabia. Fifty samples were collected from both species. The histopathological examination and PCR confirmed the IBH infection in eight samples (5 samples from chickens and 3 samples from falcons). The genomic sequence and phylogenetic analysis based on nucleotide and amino acids sequences of Saudi strains, reference fowl aviadenoviruses (FAdVs) and field viruses available in Genbank revealed that, all investigated FAdVs clustered into FAdV-2 (species D) and FAdV-...
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research
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Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research
The objectives of the present work were to carry out the molecular characterization of FAdV strains from Spanish inclusion body hepatitis cases and to study the pathogenicity and viral dynamics of these strains in specific pathogen-free (SPF) chickens. A total of 52 inclusion body hepatitis clinical cases, including 45 from broiler farms and seven from broiler breeder farms, were analysed by conventional polymerase chain reaction and sequencing targeting the FAdV hexon gene. From these, 37 strains were classified as FAdV type 8b, while the remaining 15 were classified as FAdV types 11 (n = 10), 2 (n =&...
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research
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Source: Infectious Diseases of Poverty - Category: Infectious Diseases Source Type: research
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Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research
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