Vaccination with FAdV-8a induces protection against inclusion body hepatitis caused by homologous and heterologous strains.

Vaccination with FAdV-8a induces protection against inclusion body hepatitis caused by homologous and heterologous strains. Avian Pathol. 2019 May 01;:1-30 Authors: Steer-Cope PA, Sandy JR, O'Rourke D, Scott PC, Browning GF, Noormohammadi AH Abstract Fowl aviadenoviruses (FAdV) are an important avian pathogen, responsible for several poultry diseases prevalent worldwide, including inclusion body hepatitis (IBH). FAdV intraspecies cross-protection has been clearly demonstrated, but there is little evidence that any interspecies cross-protection exists. The present study aimed to assess the inter- and intraspecies protection between three FAdV field isolates (FAdV-8a, FAdV-8b, FAdV-11) identified in association with severe IBH outbreaks. Inoculums prepared using inactivated plaque-purified virus with adjuvant Montanide™ ISA 71VG, were injected intramuscularly into three-week-old SPF chickens. At six-weeks of age the birds were challenged with 106TCID50 of homologous or heterologous virus intraperitoneally, and full post-mortem examination performed at day four post-challenge. Various tissues were examined for gross and histological lesions and assessed for presence of virus by PCR-HRM. All homologous-type vaccine/challenge groups exhibited protection against IBH lesions with no virus detected in the tissues. Unvaccinated groups challenged with virus showed evidence of FAdV-induced lesions, however FAdV-8a demonstrated lower pathogeni...
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research