Leishmania braziliensis Infection Enhances Toll-Like Receptors 2 and 4 Expression and Triggers TNF- α and IL-10 Production in Human Cutaneous Leishmaniasis

This study was carried out in accordance with the recommendations of Institutional Review Board of the Federal University of Bahia, Brazil, with written informed consent from all subjects. All subjects gave written informed consent in accordance with the Declaration of Helsinki. The protocol was approved by the Institutional Review Board of the Federal University of Bahia, Brazil (approval number 693.111). Human Blood Samples and Preparation of Peripheral Blood Cells Peripheral blood mononuclear cells (PBMC) were separated from heparinized venous blood by Ficoll-Hypaque gradient centrifugation. Cells were then washed in saline and resuspended in RPMI 1640 (supplemented with 5% of fetal calf serum, 100 U penicillin/mL, 100 ug streptomycin/ mL) (GIBCO BRL., Grand Island, NY, USA). Parasites An isolate of leishmania obtained from a skin lesion of a CL patient from Corte de Pedra (MHOM/BR/LTCP11245) was characterized as L. brazilensis using PCR and multicolus enzyme electrophoresis (Cupolillo et al., 1994). Parasites were initially grown in biphasic medium (NNN). After isolation, the parasite was cryopreserved in liquid nitrogen. The parasites selected for this study had not been previously passaged in liquid culture medium. After selection, the parasites were expanded in complete Schneider's medium (Aldrch Sigma, St. Louis, MO) supplemented with 10% fetal bovine serum (FBS) (Gilco BRL) and 2% sterile urine. For in vitro infection of PBMC, the promastigotes in the sta...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research

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Authors: Xu J, Wang X, Xiao H, Xu J Abstract BACKGROUND To determine the difference in size-specific dose estimates (SSDEs), separately based on effective diameter (deff) and water equivalent diameter (dw) of the central slice of the scan range in computed tomography coronary angiography (CTCA). MATERIAL AND METHODS There were 134 patients who underwent CTCA examination, were electronically retrieved. SSDEs (SSDEdeff and SSDEdw) were calculated using 2 approaches: deff and dw. The median SSDEs and mean absolute relative difference of SSDEs were calculated. Linear regression model was used to assess the absolute rel...
Source: Medical Science Monitor - Category: Research Tags: Med Sci Monit Source Type: research
Resveratrol Protects the Myocardium in Sepsis by Activating the Phosphatidylinositol 3-Kinases (PI3K)/AKT/Mammalian Target of Rapamycin (mTOR) Pathway and Inhibiting the Nuclear Factor-κB (NF-κB) Signaling Pathway. Med Sci Monit. 2019 Dec 06;25:9290-9298 Authors: Shang X, Lin K, Yu R, Zhu P, Zhang Y, Wang L, Xu J, Chen K Abstract BACKGROUND Sepsis combined with myocardial injury is an important cause of septic shock and multiple organ failure. However, the molecular mechanism of sepsis-induced myocardial dysfunction has not yet been thoroughly studied. Resveratrol has been an important rese...
Source: Medical Science Monitor - Category: Research Tags: Med Sci Monit Source Type: research
By scanning static, not moving, objects along both the horizontal and vertical axes instead of one, structured light illumination achieves ...
Source: Optics Letters - Category: Physics Authors: Source Type: research
This study set out to bolster morphological and molecular datasets of marine gregarine apicomplexans. Gregarines were sampled from the Sea of Japan and Northwest Pacific from cirratuliform polychaetes (Acrocirridae, Cirratulidae, and Flabelligeridae), as well as sipunculids. Trophozoites (feeding stages) were gathered for identification using light microscopy, scanning electron microscopy, and transmission electron microscopy. Cells were also collected for molecular phylogenetic analysis using 18S rDNA and 28S rDNA. As a result, three new species of Selenidium, S. planusae n. sp., S. validusae n. sp., and S. pyroidea n. sp...
Source: Protist - Category: Molecular Biology Source Type: research
Authors: Babaer D, Zheng M, Ivy MT, Zent R, Tiriveedhi V Abstract Previous phase I DNA-vaccine based clinical trials using Mammaglobin-A (Mam-A), a human breast tumor associated antigen (TAA), demonstrated that this agent was safe and efficient at treating patients with stage IV breast cancer. The long-term success of cancer vaccines is limited by the diminished expression of human leukocyte antigen (HLA) class I molecules in the tumor microenvironment. The current study assessed the impact of various selenocompounds on the expression of HLA class I molecules in THP-1 cells, an apparent proficient antigen that pres...
Source: Oncology Letters - Category: Cancer & Oncology Tags: Oncol Lett Source Type: research
Authors: Zhang C, Xiong X, Li Y, Huang K, Liu L, Peng X, Weng W Abstract Neuroblastoma (NB) is one of the most common extracranial, solid, pediatric malignancies. Despite improvements in conventional therapies, including surgery, chemotherapy and radiation therapy, the prognosis of stage IV NB remains poor, indicating that novel treatment strategies are required. Immunotherapies, such as anti-GD2 monoclonal antibodies, used alone or in combination with cytokines, and peripheral blood mononuclear cells or cord blood mononuclear cells (CBMNCs), have been indicated to cause NB cell death and to prolong patient surviva...
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Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research
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Source: Polymer Testing - Category: Chemistry Source Type: research
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Source: Colloids and Surfaces A: Physicochemical and Engineering Aspects - Category: Chemistry Source Type: research
Publication date: 7 December 2019Source: New Scientist, Volume 244, Issue 3259Author(s):
Source: New Scientist - Category: Science Source Type: research
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