A Multi-Stage Plasmodium vivax Malaria Vaccine Candidate Able to Induce Long-Lived Antibody Responses Against Blood Stage Parasites and Robust Transmission-Blocking Activity

This study including human samples was carried out in accordance with the recommendations of the ICH/GCP guidelines, Comité de Etica para Investigación con Humanos, Centro Internacional de Vacunas (CECIV, Cali, Colombia), and the protocol approved by the CECIV. All subjects gave written informed consent in accordance with the Declaration of Helsinki. All animal protocols that include experimental animal procedures using mice and NHP were carried out in accordance with the US Animal Welfare Act and approved by the Emory University's Institutional Animal Care and Use Committee and followed accordingly. Design and Biochemical Characterization of the P. vivax Chimeric Pvs25-MSP1 Protein The 861 bp synthetic gene encoding the chimeric P. vivax merozoite surface protein 1 protein (cPvMSP1) used for these studies has been previously described (Fonseca et al., 2016). A 546 bp synthetic gene encoding Pvs25 (codon optimized for expression in E. coli) was produced by Geneart (Regensburg, Germany). The sequence for the synthetic gene was derived from the Salvador I strain (XP_001608460; A23 to L195), which does not include its signal peptide and the GPI anchor. A sequence encoding the peptide MAVD was added upstream of the amino-terminal A23 for protein expression. The synthetic gene was subcloned into a pET24d(+) vector. For the production of the synthetic gene encoding the bifunctional chimeric protein, the chimeric PvMSP1 plasmid construct was digested with Xh...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research

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Date: Tuesday, 01 07, 2020; Speaker: Daniel Neafsey, Assistant Professor, Harvard T.H. Chan School of Public Health; 5625 Fishers Lane; 5th Floor Conference Room
Source: NIH Calendar of Events - Category: American Health Source Type: events
Review recent changes in ACIP's recommendations for pneumococcal conjugate vaccine use in older adults.Morbidity &Mortality Weekly Report
Source: Medscape Allergy Headlines - Category: Allergy & Immunology Tags: Public Health & Prevention Journal Article Source Type: news
In this study, sitagliptin nanoparticles (sizes ranging from 210 to 618 nm) were developed. The bioadhesion properties of the nanoparticles, as well as the swelling of the nanoparticles on the mucus membrane aided in sustained drug release. The pattern of drug release was in accordance with the Peppas model. Fourier-transform infrared (FTIR) spectroscopy demonstrated that there were no significant interactions between sitagliptin and chitosan. Differential scanning calorimetry (DSC) results showed an absence of drug peaks due to the fact that the drug was present in an amorphous state. Mucoadhesive nanoparticles wer...
Source: Biomed Res - Category: Research Authors: Tags: Biomed Res Int Source Type: research
Conclusion: Condylar tissue changes point to the osteoclastic activity in the posterior region of the condyle. These adaptive changes point to bone resorption in the posterior condyle. Type II collagen and VEGF contribute to the MCC remolding induced by the TIPD. The ultrastructural changes in the posterior condylar area in response to mechanical stresses are recoverable at the initial stage. PMID: 31815128 [PubMed - in process]
Source: Biomed Res - Category: Research Authors: Tags: Biomed Res Int Source Type: research
Abstract For bone tissue engineering, the porous scaffold should provide a biocompatible environment for cell adhesion, proliferation, and differentiation and match the mechanical properties of native bone tissue. In this work, we fabricated porous polyetherimide (PEI) scaffolds using a three-dimensional (3D) printing system, and the pore size was set as 800 μm. The morphology of 3D PEI scaffolds was characterized by the scanning electron microscope. To investigate the mechanical properties of the 3D PEI scaffold, the compressive mechanical test was performed via an electronic universal testing system. F...
Source: Biomed Res - Category: Research Authors: Tags: Biomed Res Int Source Type: research
Psychiatric Services, Ahead of Print.
Source: Psychiatric Services - Category: Psychiatry Authors: Source Type: research
CONCLUSIONS In a rat model of maternal PCOS, exposure to androgens in utero resulted in dysbiosis of the intestinal microbiota and metabolic disorders of the newborn female rats. PMID: 31815927 [PubMed - in process]
Source: Medical Science Monitor - Category: Research Tags: Med Sci Monit Source Type: research
With each single X-ray pulse having its own characteristics, understanding the individual property of each X-ray free-electron laser (XFEL) pulse is essential for its applications in probing and manipulating specimens as well as in diagnosing the lasing performance. Intensive research using XFEL radiation over the last several years has introduced techniques to characterize the femtosecond XFEL pulses, but a simple characterization scheme, while not requiring ad hoc assumptions, to address multiple aspects of XFEL radiation via a single data collection process is scant. Here, it is shown that single-particle diffraction pa...
Source: Journal of Synchrotron Radiation - Category: Physics Authors: Tags: X-ray free-electron lasers single-particle diffraction pulse energy coherence beam profile research papers Source Type: research
Publication date: Available online 9 December 2019Source: Enzyme and Microbial TechnologyAuthor(s): Liuyan Gu, Haibo Yuan, Xueqin Lv, Guangsheng Li, Rigang Cong, Jianghua Li, Guocheng Du, Long LiuAbstract5-Methylpyrazine-2-carboxylic acid (MPCA) is an important pharmaceutical intermediate and is used in the production of hypoglycemic agents and lipid-lowering drugs. This work aimed to develop a whole-cell biocatalytic process for the efficient synthesis of MPCA from 2, 5-dimethylpyrazine (DMP). Firstly, a whole-cell biocatalyst Escherichia coli strain was constructed by plasmid-based expression of xylene monooxygenase (XMO...
Source: Enzyme and Microbial Technology - Category: Biotechnology Source Type: research
Publication date: Available online 9 December 2019Source: MethodsAuthor(s): Luke Marr, David J. Clark, Jeffrey J. HayesAbstractThe assembly of DNA into nucleosomes and higher order chromatin structures serves not only as a means of compaction but also organizes the genome to facilitate crucial processes such as cell division and regulation of gene expression. Chromatin structure generally limits access to DNA, with the accessibility of DNA in chromatin being regulated through post translational modification of the histone proteins as well as the activity of chromatin remodeling proteins and architectural chromatin factors....
Source: Methods - Category: Molecular Biology Source Type: research
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