Lynx Prototoxins: Roles of Endogenous Mammalian Neurotoxin-Like Proteins in Modulating Nicotinic Acetylcholine Receptor Function to Influence Complex Biological Processes

In this study, β2∗ nAChR-containing receptors and lynx1 were identified using pull down studies from the mouse brain (Nissen et al., 2018), while ws-lynx1, SLURP-1 and SLURP-2 has been successfully mixed with human brain tissue to pull down multiple nAChR subtypes (Lyukmanova et al., 2016a,b; Thomsen et al., 2016). To support the in vivo interaction studies, a lynx1KO phenotype was ameliorated by crossing these mice into mutant mice null for α7 and β2 nAChR genes (Miwa et al., 2006). These in vivo studies provide an important biological context for the interactions observed within in vitro systems. It should be noted that, even within brain tissues, many of these interactions may take place in the cytoplasm, and may therefore be independent of gating functions on the mature receptor pentamers at the cell surface. Receptor number can be reduced via the co-expression of prototoxin (Puddifoot et al., 2015; Wu et al., 2015; George et al., 2017), suggesting an effect on receptor retention or slowing of receptor maturation. Additional studies have indicated that the receptor complexes that escape to the cell surface may form complexes prototoxins (Nichols et al., 2014; Puddifoot et al., 2015; Wu et al., 2015), retaining their modulatory functions. Except in limited cases, however (Wu et al., 2015; George et al., 2017), prototoxins have been demonstrated to retain functional effects even when they have been cleaved from the GPI-anchor using PI-PLC, suggestive...
Source: Frontiers in Pharmacology - Category: Drugs & Pharmacology Source Type: research

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Abstract The authors have observed that the function linking health outcomes with exposure to particulate-matter (PM) follows a biphasic pattern. It peaks around levels of PM10≤100 μg/m3, then weakens and rises again at PM10 levels in the range of hundreds. This could be due to a different nature of PM, the first peak reflecting a stronger anthropogenic and the second - weaker non-anthropogenic particles' effect. The current analysis is focused at the biphasic pattern on the association between PM levels with BG and asthma exacerbations. Pollutants were assessed by local monitoring stations and a satelliteba...
Source: Chemosphere - Category: Chemistry Authors: Tags: Chemosphere Source Type: research
This study provided new insights into parameters affecting As volatilization with As efflux being the main limiting factor for As methylation and subsequent volatilization in many microorganisms. PMID: 31726527 [PubMed - in process]
Source: Chemosphere - Category: Chemistry Authors: Tags: Chemosphere Source Type: research
Abstract Over twenty thousand persons rely on water from Atuwara River for drinking and other domestic purposes, hence the need to ascertain the human health risk inherent in such practice. Seventy-two water samples were collected from River Atuwara during the dry and wet seasons of 2018, and the concentration of heavy metals (Pb, As, Ni, Cr, Zn, Cu, and Cd) were measured using ICP-OES. A newly developed human health risk assessment method, HHRISK code was used to estimate the health risks associated with consumption of water from Atuwara River. Results obtained revealed that the concentration of heavy metals in t...
Source: Chemosphere - Category: Chemistry Authors: Tags: Chemosphere Source Type: research
Abstract In this research, the continuous physiological changes of zebrafish (Danio rerio) in 0.1 μg/L thallium (Tl) in 15 days were investigated. The results showed that Tl(I) stress had a significant positive linear correlation with zebrafish ammonia nitrogen excretion (ANE) (p 
Source: Chemosphere - Category: Chemistry Authors: Tags: Chemosphere Source Type: research
This study demonstrated long-term performance of the FO system at water recovery of 90% and with membrane cleaning by NaOH solution. PMID: 31726606 [PubMed - in process]
Source: Chemosphere - Category: Chemistry Authors: Tags: Chemosphere Source Type: research
Abstract There is limited information about pesticide contamination in Iran's agricultural land, particularly in plains producing exportable fruits. The aim of this investigation was to evaluate the concentration of organochlorine pesticides (OCPs) including hexachlorocyclohexane (HCH), heptachlor, dichloro-diphenyl-trichloroethane (DDT), chlordane (CHL), and their isomers compounds in agricultural soils of southern Iran. A total of 28 topsoil samples were collected from agricultural lands of Dalaki and Shabankare areas, Bushehr, Iran. In Dalaki area, the mean value of ΣHCH (α, β, γ, δ...
Source: Chemosphere - Category: Chemistry Authors: Tags: Chemosphere Source Type: research
Conclusions: Similar to previous studies, our results suggest that patients suffering from CLBP differ with regard to the magnitude of mental burden and the presence of physical impairment. These differences ascertain the need for precise targeting of treatment for CLBP. Inpatient pain centers therefore should offer different multimodal therapy pathways and integrate a meaningful triage, taking into account the multifaceted nature of CLBP based on sophisticated knowledge about forms, differences, and relationships among the biopsychosocial components of CLBP. PMID: 31728134 [PubMed - in process]
Source: GMS German Medical Science - Category: General Medicine Tags: Ger Med Sci Source Type: research
Publication date: Available online 16 November 2019Source: Life SciencesAuthor(s): Hui-Mei Wu, Qiu-Meng Xie, Cui-Cui Zhao, Juan Xu, Xiao-Yun Fan, Guang-He FeiAbstractAimsBoth CpG oligodeoxynucleotide (CpG-ODN) and melatonin have been reported to induce Th1 response and contribute to allergic asthma resistance. Here, we aimed to reveal how they confer such effect as well as whether they crosstalk with each other.Main methodsSix-week-old Female C57BL/6 mice were challenged by OVA to induce allergic airway inflammation, and were treated with CpG-ODN, CpG-ODN plus Luzindole or melatonin respectively. Bronchoalveolar lavage flu...
Source: Life Sciences - Category: Biology Source Type: research
Publication date: Available online 16 November 2019Source: Life SciencesAuthor(s): Yalda Jahanbani, Soudabe Davaran, Maryam Ghahremani-Nasab, Leili Aghebati-Maleki, Mehdi YousefiAbstractTissue engineering and the use of scaffolds have shown high therapeutic potentialities about male and female infertility. Nowadays, many couples are suffering from infertility problems. There are different causes for infertility including chemotherapy (for male and female), uterine injuries, and intrauterine adhesions. Extra-cellular matrix in tissue engineering provides a supportive medium for blood or lymphatic vessels making it a suitabl...
Source: Life Sciences - Category: Biology Source Type: research
Publication date: Available online 15 November 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Mathieu Ligibel, Charles Moore, Robert Bruccoleri, Radka SnajdrovaAbstractChiral β-hydroxy α-amino acid structural motifs are interesting and common synthons present in multiple APIs and drug candidates. To access these chiral building blocks either multistep chemical syntheses are required or the application of threonine aldolases, which catalyze aldol reactions between an aldehyde and glycine. Bioinformatics tools have been utilized to identify the gene encoding threonine aldolase ...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - Category: Biochemistry Source Type: research
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