Non-enzymatic sensing of dopamine by localized surface plasmon resonance using carbon dots-functionalized gold nanoparticles

Publication date: Available online 30 April 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Mojtaba Amiri, Shayessteh Dadfarnia, Ali Mohammad Haji Shabani, Sodeh SadjadiAbstractA highly selective, sensitive, and rapid colorimetric sensor for the determination of dopamine (DA) was developed using the color change of S-doped carbon dots functionalized gold nanoparticles (S-CDs@Au NPs). The base of the method is the formation of a complex between the amine groups of dopamine with carboxylic groups of S-CDs@Au NPs followed by their aggregation with Fe3+ ions which acts as the linkers causing a red shift from 520 to 670 nm in the localized surface plasmon peak of S-CDs@Au NPs. The ratio of absorbance intensity at 670 nm to 520 nm was monitored as the analytical signal for determination of dopamine. The parameters affecting the analytical signal including reaction time, solution pH, the concentration of Au NPs and concentration of Fe3+ were optimized. At optimized conditions, the calibration curve was linear in the concentration range of 0.81–16.80 µM of dopamine. The detection and quantification limits were 0.23 µM and 0.77 µM, respectively. The intra-day and inter-day relative standard deviation (RSDs) at 5.0 µM of DA were 3.9% and 5.6%, respectively (n = 5). The applicability of the method for determination of DA in dopamine ampoule, urine and serum human samples was investigated.Graphical abstract
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research