ATP1A1 Integrates AKT and ERK Signaling via Potential Interaction With Src to Promote Growth and Survival in Glioma Stem Cells

In this study, to improve our understanding of the role of ATP1A1 in the malignant phenotype and pathogenesis of GSCs, we evaluated ATP1A1 expression in GBMs of different grades and in two primary GSC lines established from human GBM tissues. We evaluated the role of ATP1A1 in GSC growth, its interactions with Src, and the activation of the ERK1/2 and AKT pathways. Our results revealed that ATP1A1 acts as an oncogene in our GSC models and targeting ATP1A1/Src may suppress GSC proliferation and growth. Materials and Methods Cell Isolation and Culture Human brain GBM tissues were from pathologically confirmed surgical specimens and normal human brain tissue was resected from non-neoplastic brain tissue due to hemorrhage, and them were collected at the Department of Neurosurgery of The First Affiliated Hospital of Chongqing Medical University. Primary cell cultures of GBM were established from the fresh GBM tissues. Briefly, tumors were immediately dissected and enzymatically dissociated into single cells, and red blood cells were lysed using PBS/water (1:3, v/v). GSCs were obtained through culturing the cells in serum-free medium (SFM) supplemented with DMEM/F12, 20 ng/ml basic fibroblast growth factor (bFGF, PeproTech, Rocky Hill, NJ, USA), 20 ng/ml epidermal growth factor (EGF, PeproTech, Rocky Hill, NJ, USA), B27 supplement (0.5 × ; Invitrogen, Carlsbad, CA, USA), and 10 ng/ml leukemia inhibitory factor (LIF). Differentiated GBM cells were obtained by culturing the ...
Source: Frontiers in Oncology - Category: Cancer & Oncology Source Type: research

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