ATP1A1 Integrates AKT and ERK Signaling via Potential Interaction With Src to Promote Growth and Survival in Glioma Stem Cells
In this study, to improve our understanding of the role of ATP1A1 in the malignant phenotype and pathogenesis of GSCs, we evaluated ATP1A1 expression in GBMs of different grades and in two primary GSC lines established from human GBM tissues. We evaluated the role of ATP1A1 in GSC growth, its interactions with Src, and the activation of the ERK1/2 and AKT pathways. Our results revealed that ATP1A1 acts as an oncogene in our GSC models and targeting ATP1A1/Src may suppress GSC proliferation and growth. Materials and Methods Cell Isolation and Culture Human brain GBM tissues were from pathologically confirmed surgical specimens and normal human brain tissue was resected from non-neoplastic brain tissue due to hemorrhage, and them were collected at the Department of Neurosurgery of The First Affiliated Hospital of Chongqing Medical University. Primary cell cultures of GBM were established from the fresh GBM tissues. Briefly, tumors were immediately dissected and enzymatically dissociated into single cells, and red blood cells were lysed using PBS/water (1:3, v/v). GSCs were obtained through culturing the cells in serum-free medium (SFM) supplemented with DMEM/F12, 20 ng/ml basic fibroblast growth factor (bFGF, PeproTech, Rocky Hill, NJ, USA), 20 ng/ml epidermal growth factor (EGF, PeproTech, Rocky Hill, NJ, USA), B27 supplement (0.5 × ; Invitrogen, Carlsbad, CA, USA), and 10 ng/ml leukemia inhibitory factor (LIF). Differentiated GBM cells were obtained by culturing the ...
Publication date: Available online 2 June 2020Source: Pathology - Research and PracticeAuthor(s): Hyeongjoo Kim, Taewan Kim, Gunn Jaygal, Jongsoo Woo, Chang-Jin Kim, Moo-Jun Baek, Dongjun Jeong
Publication date: Available online 2 June 2020Source: Pathology - Research and PracticeAuthor(s): Qian Wu, Weiya Wang, Ping Zhou, Yiyun Fu, Ying Zhang, Yang W. Shao, Lili Jiang
ABSTRACT Objective To assess the outcome of an educational nutritional intervention in the quality of diet of women with breast cancer in adjuvant treatment. Methods Women with breast cancer and admitted for surgical treatment were divided in an intervention group (n=18) and a comparison group (n=78), and participated in a nonrandomized clinical trial. Participants were assessed before and after the treatment and/or intervention. A food frequency questionnaire was applied and the quality of diet was calculated using the Brazilian Healthy Eating Index Revised. The educational nutritional intervention lasted 12 months and wa...
Publication date: Available online 2 June 2020Source: Nutrition, Metabolism and Cardiovascular DiseasesAuthor(s): Qi Zhao, Ting-Yu Zhang, Yu-Jing Cheng, Yue Ma, Ying-Kai Xu, Jia-Qi Yang, Yu-Jie Zhou
Publication date: Available online 2 June 2020Source: Nutrition, Metabolism and Cardiovascular DiseasesAuthor(s): Zhaoxie Tang, Saijun Huang, Rui Ma, Hao Zheng, Yanna Zhu
On the heels of studies showing hydroxychloroquine doesn't help patients in the hospital with Covid-19, a new study -- the first of its kind -- shows the drug doesn't work to prevent infection with the virus, either.
The United States should have 100 million doses of one candidate coronavirus vaccine by the end of the year, Dr. Anthony Fauci, the director of the National Institute of Allergy and Infectious Diseases and a member of the White House Coronavirus Task Force, said Tuesday.
The Trump administration chose 5 companies as the most likely to produce a vaccine. The tally of new cases is rising in the U.S., partly because of expanded testing. Italy ends travel restrictions.
Italian public health officials and the World Health Organization warn there's no evidence to support claims that SARS-CoV-2 is weakening, as some Italian clinicians have reported.WebMD Health News
Researchers say that a malaria drug President Donald Trump took to try to prevent COVID-19 proved ineffective for that in the first large, high-quality study to test it in health workers and others closely exposed to people with the disease
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