High-Content Screening Identifies Vanilloids as a Novel Class of Inhibitors of NET Formation

This study was approved by the Regional Ethical Committee (Comitato Etico Regionale) on October 24, 2014, under the supervision of the Italian Ministry of Health. Written informed consent was obtained from all donors using a form that was also approved by the same Ethical Committee. Author Contributions ES, RB, and NP conceptualization. ES, EP, and RB investigation and validation. ES, RB, and NP writing. GMG and NP funding acquisition. GMG and NP resources. Conflict of Interest Statement The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. Acknowledgments The authors would like to thank Antonio De Flora and Enrico Millo for helpful discussion and Anna Capurro for English language revision. This work was supported by grants from the Italian Ministry of Health through Cinque per mille and Ricerca Corrente (Linea1) from the Italian Ministry of Health, and from ERA-Net for Research Programmes on Rare Diseases (01GM1601). Supplementary Material The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fimmu.2019.00963/full#supplementary-material Supplementary Figure 1. Induction of NET formation in human neutrophils. Confocal analysis of morphological changes occurring in Hoechst 33342-stained nuclei of human neutrophils freshly isolated from healthy donor, following treatment with PMA (for 210 min) ...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research