A photo-triggered conjugation approach for attaching RGD ligands to biodegradable mesoporous silica nanoparticles for the tumor fluorescent imaging

Publication date: Available online 29 April 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Xinyi Xu, Hanrui Li, Ke Li, Qi Zeng, Yin Liu, Yun Zeng, Dan Chen, Jimin Liang, Xueli Chen, Yonghua ZhanAbstractFluorescent probes conjugated to peptide or antibody directing groups, that exhibit high signal to background ratios, have been widely used to image tumors and monitor their growth. A photo-triggered cycloaddition reaction between the arginine-glycine-aspartic acid -N-ɛ-acryllysine (RGD-Acrk) peptides and the tetrazole compounds bound to the surface of biodegradable mesoporous silica nanoparticles (bMSN) has been used to construct a fluorescent nanoprobe (bMSN@T2-RGD-Acrk), which was shown fluorescent emission at 550 nm and could selectively image the 4 T1 cells and breast cancer. This means that the bMSN@T2-RGD-Acrk nanoprobe made by photo-triggered conjugation approach is the promising fluorescent imaging agents for visualizing tumors. Thus, the photo-triggered one-spot reaction can give a stable crosslinker in a biocompatible manner for bioconjugation with nanoparticles and produce a fluorescent group that is suitable for imaging in vivo.Graphical AbstractA photo-triggered click reaction has been used to prepare a novel RGD conjugated fluorescence bMSN@T2-RGD-Acrk nanoprobe that exhibits excellent tumor targeting ability and low toxicity, which means it is a highly promising imaging agent for the diagnosis of cancer and monitoring tumor growth...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - Category: Nanotechnology Source Type: research