Antidiabetic drugs and their nanoconjugates repurposed as novel antimicrobial agents against Acanthamoeba castellanii.
Antidiabetic drugs and their nanoconjugates repurposed as novel antimicrobial agents against Acanthamoeba castellanii.
J Microbiol Biotechnol. 2019 Apr 22;:
Authors: Anwar A, Siddiqui R, Raza Shah M, Khan NA
Abstract
Acanthamoeba castellanii belonging to the T4 genotype may cause fatal brain infection known as granulomatous amoebic encephalitis, and vision-threatening eye infection Acanthamoeba keratitis. The aim of this study was to evaluate the antiamoebic effects of three clinically available antidiabetic drugs Glimepiride, Vildagliptin and Repaglinide against A. castellanii belonging to the T4 genotype. Furthermore, these drugs were attempted to conjugate with silver nanoparticles (AgNPs) to enhance antiamoebic effects. Amoebicidal, encystation, excystation, and host cells cytotoxicity assays were performed to unravel antiacanthamoebic effects. Vildagliptin conjugated silver nanoparticles (Vgt-AgNPs) were synthesized which were characterized by spectroscopic techniques and atomic force microscopy. All three drugs showed antiamoebic effects against A. castellanii and significantly blocked the encystation. These drugs also showed significant cysticidal effects and reduced host cells cytotoxicity caused by A. castellanii. Moreover, Vildagliptin coated silver nanoparticles were successfully synthesized and are shown to enhance its antiacanthamoebic potency at significantly reduced concentration. The repurposed application of tested a...
Source: Journal of Microbiology and Biotechnology - Category: Biotechnology Authors: Anwar A, Siddiqui R, Raza Shah M, Khan NA Tags: J Microbiol Biotechnol Source Type: research
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