Differentiation of Bacillus thuringiensis From Bacilluscereus Group Using a Unique Marker Based on Real-Time PCR

Conclusion Since B. cereus group are highly similar in biochemical as well as genetic profiles, a new biomarker was developed for identifying and distinguishing B. thuringiensis from the closely related group. The performance of XRE was compared with cry2 gene and artificially contaminated samples were also tested. Compared with cry2, XRE gene was observed to be efficiently accurate in the identification of B. thuringiensis. Further, the developed real-time PCR using XRE successfully identified B. thuringiensis and it could be used to quantify cell numbers with the generated standard curve. Author Contributions The experiments were conceived and designed by SW, RC, YG-J, and DH-O. SW, BJ-P, FF, MC, and DS-P performed the experiments. BJ-P and SH-K discussed the experiments and results. SW and RC wrote the manuscript. Funding This research work was supported by the Ministry of Agriculture, Food and Drug Safety (Grant No. 14162MFDS085) of Republic of Korea and Kangwon National University in 2015. SW was grateful for the financial support from the China Scholarship Council (CSC No: 201408260054). The authors would like to thank Hyun-Ah Lee at the Central Laboratory of the Kangwon National University for the technical support. RC is grateful for the financial support from the National Research Foundation of Korea (NRF) 2018007551. Conflict of Interest Statement The authors declare that the research was conducted in the absence of any commercial or financial relationships t...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research

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Publication date: Available online 18 January 2020Source: The American Journal of SurgeryAuthor(s): Asishana A. Osho, Muath M. Bishawi, Elbert E. Heng, Ejiro Orubu, Aaron Amardey-Wellington, Mauricio A. Villavicencio, Masaki FunamotoAbstractBackgroundFailure to Rescue (FTR) is a valuable surgical quality improvement metric. The aim of this study is to assess the relationship between center volume and FTR following lung transplantation.MethodsUsing the database of the United Network for Organ Sharing (UNOS) all adult, primary, isolated lung recipients in the United States between May 2005 and March 2016 were identified. FTR...
Source: The American Journal of Surgery - Category: Surgery Source Type: research
ConclusionOverall, these guidelines provide consistent recommendations on who should receive breast reconstruction education, who is a candidate for postmastectomy breast reconstruction, and the appropriate timing of reconstruction and extent of mastectomy. Future updates from all should focus on expanding to include alloplastic and autologous forms of reconstruction and should include a broad scope of relevant questions.
Source: European Journal of Surgical Oncology (EJSO) - Category: Surgery Source Type: research
CONCLUSIONS: The GSDS-26 is a preliminary validated multidimensional scale for better identifying depression in men and may be suitable for routine use after further validation. PMID: 31952089 [PubMed - as supplied by publisher]
Source: Psychiatrische Praxis - Category: Psychiatry Tags: Psychiatr Prax Source Type: research
CONCLUSION: The positive evaluation and the potential for improvements of the PNP program can support its advancement (e. g. enrolment, billing). PMID: 31952088 [PubMed - as supplied by publisher]
Source: Psychiatrische Praxis - Category: Psychiatry Tags: Psychiatr Prax Source Type: research
CONCLUSION: Shame needs to be viewed in context of gender roles, status, and their interaction. Future studies should investigate the influence of mental health literacy. PMID: 31952086 [PubMed - as supplied by publisher]
Source: Psychiatrische Praxis - Category: Psychiatry Tags: Psychiatr Prax Source Type: research
CONCLUSIONS: In the population studied mental illness alone was not a sufficient predictor for suicide. Rather, an interaction between mental illness, psychosocial crisis and other factors may explain and predict suicides. Suicide prevention should better address the needs of relatives and family doctors and offer specific low-threshold services. PMID: 31952085 [PubMed - as supplied by publisher]
Source: Psychiatrische Praxis - Category: Psychiatry Tags: Psychiatr Prax Source Type: research
CONCLUSIONS: Psychotherapeutic work in rural areas should be better incentivized. Interventions to reduce east-west inequalities in the density of service provision seem to be necessary. PMID: 31952084 [PubMed - as supplied by publisher]
Source: Psychiatrische Praxis - Category: Psychiatry Tags: Psychiatr Prax Source Type: research
CONCLUSIONS: Elements of the guideline should be evaluated in relation to the preferred treatment method. Aspects related to the psychotherapeutic work with patients seem to be especially significant. PMID: 31952083 [PubMed - as supplied by publisher]
Source: Psychiatrische Praxis - Category: Psychiatry Tags: Psychiatr Prax Source Type: research
Publication date: Available online 18 January 2020Source: Journal of Hospital InfectionAuthor(s): Vincent C.C. Cheng, Shuk-Ching Wong, Kelvin K.W. To, P.L. Ho, Kwok-Yung Yuen
Source: Journal of Hospital Infection - Category: Infectious Diseases Source Type: research
Conclusion: Pneumatic posterior capsulorhexis is a new effective technique for the treatment of primary PCO in dense cataract and for prevention of postoperative PCO with the good visual outcomes and minimal complications. This trial is registered with NCT04007965. PMID: 31949950 [PubMed]
Source: Journal of Ophthalmology - Category: Opthalmology Tags: J Ophthalmol Source Type: research
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