Differentiation of Bacillus thuringiensis From Bacilluscereus Group Using a Unique Marker Based on Real-Time PCR

Conclusion Since B. cereus group are highly similar in biochemical as well as genetic profiles, a new biomarker was developed for identifying and distinguishing B. thuringiensis from the closely related group. The performance of XRE was compared with cry2 gene and artificially contaminated samples were also tested. Compared with cry2, XRE gene was observed to be efficiently accurate in the identification of B. thuringiensis. Further, the developed real-time PCR using XRE successfully identified B. thuringiensis and it could be used to quantify cell numbers with the generated standard curve. Author Contributions The experiments were conceived and designed by SW, RC, YG-J, and DH-O. SW, BJ-P, FF, MC, and DS-P performed the experiments. BJ-P and SH-K discussed the experiments and results. SW and RC wrote the manuscript. Funding This research work was supported by the Ministry of Agriculture, Food and Drug Safety (Grant No. 14162MFDS085) of Republic of Korea and Kangwon National University in 2015. SW was grateful for the financial support from the China Scholarship Council (CSC No: 201408260054). The authors would like to thank Hyun-Ah Lee at the Central Laboratory of the Kangwon National University for the technical support. RC is grateful for the financial support from the National Research Foundation of Korea (NRF) 2018007551. Conflict of Interest Statement The authors declare that the research was conducted in the absence of any commercial or financial relationships t...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research