Biological Characteristics of Severe Combined Immunodeficient Mice Produced by CRISPR/Cas9-Mediated Rag2 and IL2rg Mutation

Discussion In present study, severe combined immunodeficient mice were prepared by CRISPR/Cas9-mediated Rag2 and IL2rg mutation. This mice was produced from clear genetic background of BALB/c inbred strain, with homozygosity as the main characteristic. Although severe combined immunodeficient mice could also be obtained by mating Rag2 with IL2rg mutated mice (Belizário, 2009), but this mating might increase heterozygosity since Rag2 and IL2rg mutated mice were from different strains. In addition, the biological characteristics of this immunodeficient mice were studied, the mice not only developed abnormal lymphatic organs, leading to number of immune cells decreased, but also could not induce immune response even stimulated with recombinant antigen, resulting in immune response defects. Interestingly, this attenuant immune response was more susceptible to compromising tumor xenotransplantation, which made this mice was more adapted to tumor xenograft model. Gene mutation led to the production of an immunodeficiency animal. Foxn1 gene mutation brought about a T-lymphocyte-mediated cellular immune response defect of hairless nude mice (Vaidya et al., 2016), Xid gene mutations produced a B-lymphocyte-mediated humoral immune response obstacle of CBA/N mice (Szymczak et al., 2013), and beige gene mutations also produced NK cell dysfunction of beige mice (Pflumio et al., 1989). At this point, these immunodeficiency animals showed only a single immune cell disorder. Gene mu...
Source: Frontiers in Genetics - Category: Genetics & Stem Cells Source Type: research