Transcriptomic Analysis of Mecp2 Mutant Mice Reveals Differentially Expressed Genes and Altered Mechanisms in Both Blood and Brain

Transcriptomic Analysis of Mecp2 Mutant Mice Reveals Differentially Expressed Genes and Altered Mechanisms in Both Blood and BrainAlbert Sanfeliu1, Karsten Hokamp2, Michael Gill1 and Daniela Tropea1,3*1Neuropsychiatric Genetics, Department of Psychiatry, School of Medicine, Trinity Translational Medicine Institute, St James Hospital, Dublin, Ireland2Department of Genetics, School of Genetics and Microbiology, Smurfit Institute of Genetics, Trinity College Dublin, Dublin, Ireland3Department of Psychiatry, School of Medicine, Trinity College Institute for Neuroscience, Trinity College Dublin, Dublin, IrelandRett syndrome is a rare neuropsychiatric disorder with a wide symptomatology including impaired communication and movement, cardio-respiratory abnormalities, and seizures. The clinical presentation is typically associated to mutations in the gene coding for the methyl-CpG-binding protein 2 (MECP2), which is a transcription factor. The gene is ubiquitously present in all the cells of the organism with a peak of expression in neurons. For this reason, most of the studies in Rett models have been performed in brain. However, some of the symptoms of Rett are linked to the peripheral expression of MECP2, suggesting that the effects of the mutations affect gene expression levels in tissues other than the brain. We used RNA sequencing in Mecp2 mutant mice and matched controls, to identify common genes and pathways differentially regulated across different tissues. We performed our ...
Source: Frontiers in Psychiatry - Category: Psychiatry Source Type: research

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Conclusions: CC participants reached target AUC0-12 quicker. Larger studies are required to test clinical efficacy.
Source: Therapeutic Drug Monitoring - Category: Drugs & Pharmacology Tags: Original Article Source Type: research
Publication date: January 2020Source: Molecular Immunology, Volume 117Author(s): Tang Li, Ru-ru Chen, Hong-peng Gong, Bin-feng Wang, Xi-xi Wu, Yue-qiu Chen, Zhi-ming HuangAbstractInflammatory bowel disease (IBD) is an autoimmune disease characterized by an abnormal immune response. Fibrinogen-like protein 2 (FGL2) is known to have immunoregulatory and anti-inflammatory activity. The level of FGL2 is elevated in patients with IBD; however, its comprehensive function in IBD is almost unknown. In our study, we explored the effect of FGL2 on dextran sulfate sodium (DSS)-induced colitis in mice and on NF-κB signaling in i...
Source: Molecular Immunology - Category: Allergy & Immunology Source Type: research
Publication date: Available online 16 November 2019Source: Materials Research BulletinAuthor(s): Yuxia Kong, Huanyu Zhao, Longting Li, Yu Long, Jigong HaoAbstractIn this work, the effects of oxide additives on the phase structure and electrical properties of sodium bismuth titanate-based [0.94(Bi0.5Na0.5)TiO3–0.06BaTiO3 (BNBT6)] lead-free ceramics were investigated. Results showed that all oxide additives diffused into the BNBT6 lattice to form a single perovskite phase structure. The oxide additives doped into BNBT6 enhanced the electric-field-induced strain without diminishing its piezoelectric activity. With Mn ad...
Source: Materials Research Bulletin - Category: Materials Science Source Type: research
Publication date: Available online 17 November 2019Source: NeuroscienceAuthor(s): Ana R. Machado-Santos, Nuno D. Alves, Bruna Araújo, Joana S. Correia, Patrícia Patrício, António Mateus-Pinheiro, Eduardo Loureiro-Campos, João M. Bessa, Nuno Sousa, Luísa PintoAbstractAstrocytes are now known to play crucial roles in the central nervous system, supporting and closely interacting with neurons and therefore able to modulate brain function. Both human postmortem studies in brain samples from patients diagnosed with Major Depressive Disorder and from animal models of depression reported ...
Source: Neuroscience - Category: Neuroscience Source Type: research
Publication date: Available online 16 November 2019Source: Neuroscience &Biobehavioral ReviewsAuthor(s): Aleksandra Lecei, Ruud van WinkelAbstractChildhood adversity increases the risk of developing psychiatric symptoms later in life. However, the neurobiological mechanisms underlying this association are unknown. This paper reviews the current literature regarding structural and functional alterations in the hippocampus and amygdala following childhood adversity, ultimately converging into a model that proposes impaired ‘pattern separation’ as an important underlying mechanism associated with risk or resil...
Source: Neuroscience and Biobehavioral Reviews - Category: Neuroscience Source Type: research
Discussion Holoprosencephaly (HPE) is a clefting problem of the brain. “[HPE] the result of incomplete or absent midline division of the embryonic forebrain into distinct cerebral hemispheres (prosencephalon) between the 18th and 28th day after conception.” There are four distinct subtypes: Alobar – both hemispheres are completely fused and are not separated into the left and right hemispheres. There is agenesis of the corpus callosum, arrhinencephaly and a single ventricle with fused thalami. Facial features are almost always affected. Semilobar – the cerebral hemispheres are fused anteriorly bu...
Source: PediatricEducation.org - Category: Pediatrics Authors: Tags: Uncategorized Source Type: news
Publication date: Available online 17 November 2019Source: Process BiochemistryAuthor(s): Kadathur Ramachandran Ramya, Ramar Thangam, Balaraman MadhanABSTRACTHair waste is one of the solid substances rejected by the leather industry. This waste finds its way into the surroundings causing serious environmental pollution. This hair waste may be utilized for effective extraction of keratin, thereby generating value-added products with numerous applications. Thus we focusing on utilizing red sheep’s hair waste for extracting keratin by the application of different chemical treatments such as sodium hydroxide, sodium sulf...
Source: Process Biochemistry - Category: Biochemistry Source Type: research
Publication date: Available online 16 November 2019Source: Sensors and Actuators B: ChemicalAuthor(s): Xueying Pei, Fangjun Huo, Yongkang Yue, Tinggui Chen, Caixia YinAbstractCancer is a leading cause of death worldwide. The discrimination of cancer cells from normal cells is of importance for early diagnosis. Designing and synthesizing fluorescent probes that precisely target cancer cells can greatly aid treatment efforts. Biotin can target over-expressed receptors on the surface of cancer cells. In addition, the abundant free biothiols in cells play important roles in many physiological and pathological processes. We the...
Source: Sensors and Actuators B: Chemical - Category: Chemistry Source Type: research
This study used xurographic fabrication technology to rapidly fabricate a low cost miniaturized microbial fuel cell (MFC) and demonstrated its suitability to measure BOD. The miniaturized sensor could be fabricated in 10 minutes with low cost of $0.5 U.S. per device. The reaction volume was designed to be 1.8 µL to obtain faster response time. The sensor was tested using sodium acetate (NaAc) as a model BOD analyte. It could response to a wide range of BOD concentration between 20 and 490 mg/L which would cover the majority range of wastewater BOD concentration in a wastewater treatment plant. Th...
Source: Sensors and Actuators B: Chemical - Category: Chemistry Source Type: research
In this study, we investigated the role of sodium dodecylbenzene sulfonate (SDBS) to induce aggregation in equine heart muscle myoglobin (E-Mb) at pH 4.5. The aggregation was analyzed by Rayleigh scattering (RLS), turbidity, Thioflavin-T binding (ThT), and Congo red (CR) binding, circular dichroism spectroscopy and transmission electron microscopy (TEM). Turbidity and RLS analysis demarcated the aggregate were formed at effective range of SDBS concentration at pH 4.5, this led to the observation that at low concentrations (0.05 mM-1.2 mM) SDBS cause aggregation of E-Mb monomers, in contrast at higher SDBS con...
Source: Colloids and Surfaces A: Physicochemical and Engineering Aspects - Category: Chemistry Source Type: research
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