Application of colony BOXA2R-PCR for the differentiation and identification of lactic acid COCCI.

In this study, it was demonstrated that BOXA2R-PCR could effectively discriminate between Lactococcus lactis, Leuconostoc mesenteroides and Streptococcus thermophilus; differentiate Lactococcus lactis strains and subspeciate them into lactis and cremoris in a single reaction; generate unique strain fingerprints of various lactic acid bacteria (LAB species) commonly isolated from fermented dairy products, including occasional spoilage bacteria and yeasts. Furthermore, using direct colony PCR a reproducible and rapid method was developed for the differentiation and identification of lactic acid cocci. The simplicity and speed of this microbial identification method has potential practical value for dairy microbiologists, which was demonstrated through a microbiota investigation of select Australian retail dairy products. PMID: 31027784 [PubMed - in process]
Source: Food Microbiology - Category: Food Science Authors: Tags: Food Microbiol Source Type: research

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In this report, considering also the literature information, a case of G.sanguinis which is thought to cause meningitis was presented. Our case is a 39-year-old female patient with a lumboperitoneal shunt. The patient was admitted to the neurosurgery clinic with a headache and vision loss and was hospitalized in the service with a pre-diagnosis of pseudotumor cerebri. Neurological examination revealed no pathological findings. Eye examination revealed mild papillary edema, local retinal hemorrhage, and bilateral expansion in retinal vascularization. There was no pathologic findings in the brain magnetic resonance imaging. ...
Source: Mikrobiyoloji Bulteni - Category: Microbiology Tags: Mikrobiyol Bul Source Type: research
Coccidioides spp. are dimorphic fungi endemic to Central America, regions of South America and southwestern USA. Two species cause most human disease: Coccidioides immitis (primarily California isolates) and Cocc...
Source: BMC Infectious Diseases - Category: Infectious Diseases Authors: Tags: Case report Source Type: research
Publication date: Available online 14 August 2019Source: Journal of the American Society of CytopathologyAuthor(s): Dianna L. Ng, Ronald BalassanianAbstractIntroductionFine needle aspiration biopsy (FNAB) is a minimally invasive biopsy technique and is an important tool for diagnosing infectious diseases. Rapid on-site evaluation allows for triage for ancillary testing, including microbiologic cultures. We aim to determine the etiology of granulomatous inflammation diagnosed by FNAB by correlating with culture results and clinical history.Materials and methodsA 16-year retrospective review of cases diagnosed as “gran...
Source: Journal of the American Society of Cytopathology - Category: Cytology Source Type: research
International Journal of Impotence Research, Published online: 08 August 2019; doi:10.1038/s41443-019-0176-1Radical prostatectomy and simultaneous penile prosthesis implantation: a narrative review
Source: International Journal of Impotence Research - Category: Urology & Nephrology Authors: Source Type: research
Conclusions Infantile ocular trauma is a common morbidity that is underreported. Self-infliction by child's hand was found to be the main cause of corneal trauma. Cases presenting early and following up regularly till recovery have a favorable clinical course with good outcome. A high loss to follow-up indicates that awareness needs to be created among the caregivers.
Source: Pediatric Emergency Care - Category: Emergency Medicine Tags: Original Articles Source Type: research
Abstract The microbial surface components recognizing adhesive matrix molecules (MSCRAMMs) are a family of proteins that are defined by the presence of two adjacent IgG-like folded subdomains. These promote binding to ligands by mechanisms that involve major conformational changes exemplified by the binding to fibrinogen by the 'dock-lock-latch' mechanism or to collagen by the 'collagen hug'. Clumping factors A and B are two such MSCRAMMs that have several important roles in the pathogenesis of Staphylococcus aureus infections. MSCRAMM architecture, ligand binding, and roles in infection and colonization are exami...
Source: Trends in Microbiology - Category: Microbiology Authors: Tags: Trends Microbiol Source Type: research
Discussion Dengue is an important arboviral infection that affects about 40% of the world population. It is found mainly in topical and subtropical areas of the world mainly in developing countries but it range is spreading including the United States. A review of common arboviruses can be found here. It is a flaviavirus with 4 distinct serotypes named DENV-1 through DENV-4 and is spread by A. aegypti a day biting mosquito. Infection with one serotype confers immunity to that serotype but not the others. It does offer some protection for cross-infection but this only lasts a few months. Incubation period is 3-14 days with ...
Source: PediatricEducation.org - Category: Pediatrics Authors: Tags: Uncategorized Source Type: news
Publication date: Available online 26 July 2019Source: Journal of Cardiology CasesAuthor(s): Kartik Pandurang Jadhav, Praveen G PaiAbstractA 70-year-old male with a past history of coronary artery bypass grafting, presented with high-grade fever and rapidly progressive dyspnea for 3 days. He was febrile and a prominent diastolic murmur was noted in the aortic area. Transesophageal echocardiography showed severe aortic regurgitation with multiple mobile vegetations attached to both supra- and infravalvular surfaces of aortic valve. Blood cultures grew Vagococcus fluvialis which was confirmed with identification and antibiot...
Source: Journal of Cardiology Cases - Category: Cardiology Source Type: research
Authors: Manshadi SAD, Alizadeh R, Salehi M, Seifi A, Seifi M Abstract Septic arthritis (SA) remains to be a critical diagnosis for a swollen knee at the emergency department. Here, we report a rare case of bilateral knee arthritis in a 59-year-old diabetic woman who had been immobilized 5 months prior to admission. Her right knee swelling exacerbated in 10 days leading to left knee involvement. In 5 days the clear synovial tap in the first hospital turned purulent in the second hospital and empirical antibiotics get started with high WBC count, dominant neutrophils, and Gram-positive cocci in smear. Knee arthrotom...
Source: Iranian Journal of Microbiology - Category: Microbiology Tags: Iran J Microbiol Source Type: research
Coccidioidomycosis (Valley fever) is an infection caused by the soil-inhabiting molds, Coccidioides immitis or Coccidioides posadasii.2 Southern Arizona is one of the endemic region for this fungus, posing a unique challenge to the patients residing in this area. Of the 150,000 new coccidioidomycosis infections occuring annually in the United States, approximately 1% are disseminated infections and one third of these cases are often fatal.3 –5
Source: The American Journal of Medicine - Category: General Medicine Authors: Tags: Clinical Communication to the Editor Source Type: research
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