Curli fimbriae confer shiga toxin-producing Escherichia coli a competitive trait in mixed biofilms.

Curli fimbriae confer shiga toxin-producing Escherichia coli a competitive trait in mixed biofilms. Food Microbiol. 2019 Sep;82:482-488 Authors: Carter MQ, Feng D, Li HH Abstract Shiga toxin-producing Escherichia coli (STEC) is one of the most common causal agents of foodborne illness linked to fresh leafy vegetables. Here, we investigated the impact of spinach-associated microorganisms on proliferation and biofilm formation of STEC O157:H7 on stainless steel surfaces at temperatures related to produce production and postharvest processing environments. Although a proliferation of inoculated pathogen cells in spinach leaf wash water was detected at all temperatures examined, the impact of spinach-associated microorganisms on the proliferation of E. coli O157:H7 was observed at 10 °C and 26 °C, but not at 4 °C. The inhibition of E. coli O157:H7 growth by spinach-associated microorganisms indicated a competition between the pathogen and spinach indigenous microflora. A significant decrease of the pathogen population in mixed biofilms was observed only at 26 °C for curli-deficient strain MQC43, but not for curli-expressing strain MQC57. Deletion of curli genes in a curli-expressing strain resulted in a phenotype similar to that of MQC43 in mixed biofilms; however, this deficiency was rescued when curli biogenesis was restored in the curli-deletion mutant strain. Our data support that curli confer E. coli O157:H7 a competitive trait i...
Source: Food Microbiology - Category: Food Science Authors: Tags: Food Microbiol Source Type: research

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Abstract Norovirus (NoV) is the main cause of gastroenteritis outbreaks worldwide. Although NoV spreads mainly from person to person, it is estimated that a large proportion of NoV outbreaks are caused by foodborne transmission. Bivalve mollusks are one of the most important foods involved in NoV transmission to humans. Little is known about NoV prevalence in shellfish harvested and commercialized in Brazil. The aim of this study was to map, for the first time, the distribution of NoV contamination in oysters and mussels harvested and commercialized in the coast of Pernambuco state, northeast Brazil. A total of 38...
Source: Braz J Med Biol Res - Category: Research Authors: Tags: Braz J Med Biol Res Source Type: research
Publication date: Available online 22 September 2020Source: Journal of Microbiology, Immunology and InfectionAuthor(s): Chin-Chung Tseng, Wei-Hung Lin, An-Bang Wu, Ming-Cheng Wang, Ching-Hao Teng, Jiunn-Jong Wu
Source: Journal of Microbiology, Immunology and Infection - Category: Microbiology Source Type: research
Publication date: Available online 22 September 2020Source: Journal of BiotechnologyAuthor(s): Takuya Matsumoto, Mizuho Kamino, Ryosuke Yamada, Yasuhiro Konishi, Hiroyasu Ogino
Source: Journal of Biotechnology - Category: Biotechnology Source Type: research
Publication date: Available online 22 September 2020Source: Journal of Bioscience and BioengineeringAuthor(s): Yoshihiro Ojima, Hiroaki Honma, Mio Otsuka, Satsuki Matano, Masayuki Azuma
Source: Journal of Bioscience and Bioengineering - Category: Biomedical Science Source Type: research
Green Chem., 2020, Advance Article DOI: 10.1039/D0GC02395G, PaperBakht Zada, Seongjoon Joo, Chonglong Wang, Tenzin Tseten, Seong-Hee Jeong, Hogyun Seo, Jung-Hoon Sohn, Kyung-Jin Kim, Seon-Won Kim Development of bioconversion technology for efficient conversion of crude glycerol to non-natural acetins. To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Green Chem. latest articles - Category: Chemistry Authors: Source Type: research
In conclusion, our results show for the first time that CATH-B1 has several immunomodulatory activities and thereby could be an important factor in the chicken immune response.
Source: Veterinary Research - Category: Veterinary Research Source Type: research
Mucosal Immunology, Published online: 24 September 2020; doi:10.1038/s41385-020-00345-8Secretory IgA N-glycans contribute to the protection against E. coli O55 infection of germ-free piglets
Source: Mucosal Immunology - Category: Allergy & Immunology Authors: Source Type: research
This study aims to evaluate the in vitro antimicrobial, cytotoxic, and tolerance levels of Lactobacillus bulgaricus KLDS 1.0207 against two notable foodborne pathogens – Escherichia coli ATCC25922 and Staphylococcus aureus ATCC25923. Afterward, a 48 BALB/c mice-trial was used to assess its ameliorative effects on weight and serum biochemical parameters. Results showed that the cell-free supernatant (CFS) of this strain significantly inhibited both pathogens, but these effects were abolished at pH 6.5 and 7.0 (P
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Vibrio parahaemolyticus non-toxigenic strains are responsible for about 10% of acute gastroenteritis associated with this species, suggesting they harbor unique virulence factors. Zonula occludens toxin (Zot), firstly described in Vibrio cholerae, is a secreted toxin that increases intestinal permeability. Recently, we identified Zot-encoding genes in the genomes of highly cytotoxic Chilean V. parahaemolyticus strains, including the non-toxigenic clinical strain PMC53.7. To gain insights into a possible role of Zot in V. parahaemolyticus, we analyzed whether it could be responsible for cytotoxicity. However, we observed a ...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
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