Curli fimbriae confer shiga toxin-producing Escherichia coli a competitive trait in mixed biofilms.

Curli fimbriae confer shiga toxin-producing Escherichia coli a competitive trait in mixed biofilms. Food Microbiol. 2019 Sep;82:482-488 Authors: Carter MQ, Feng D, Li HH Abstract Shiga toxin-producing Escherichia coli (STEC) is one of the most common causal agents of foodborne illness linked to fresh leafy vegetables. Here, we investigated the impact of spinach-associated microorganisms on proliferation and biofilm formation of STEC O157:H7 on stainless steel surfaces at temperatures related to produce production and postharvest processing environments. Although a proliferation of inoculated pathogen cells in spinach leaf wash water was detected at all temperatures examined, the impact of spinach-associated microorganisms on the proliferation of E. coli O157:H7 was observed at 10 °C and 26 °C, but not at 4 °C. The inhibition of E. coli O157:H7 growth by spinach-associated microorganisms indicated a competition between the pathogen and spinach indigenous microflora. A significant decrease of the pathogen population in mixed biofilms was observed only at 26 °C for curli-deficient strain MQC43, but not for curli-expressing strain MQC57. Deletion of curli genes in a curli-expressing strain resulted in a phenotype similar to that of MQC43 in mixed biofilms; however, this deficiency was rescued when curli biogenesis was restored in the curli-deletion mutant strain. Our data support that curli confer E. coli O157:H7 a competitive trait i...
Source: Food Microbiology - Category: Food Science Authors: Tags: Food Microbiol Source Type: research

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Publication date: Available online 18 August 2019Source: Alzheimer's &DementiaAuthor(s): Binyin Li, Yixi He, Jianfang Ma, Pei Huang, Juanjuan Du, Li Cao, Yan Wang, Qin Xiao, Huidong Tang, Shengdi ChenAbstractObjectiveGut microbiota changes before the onset of Alzheimer's disease (AD) and the alterations could be detected in the stage of mild cognitive impairment (MCI). The findings might offer diagnostic biomarkers before the onset of dementia.BackgroundAD is the most common cause of dementia, and MCI is the predementia state. Recent studies suggest the alterations in the gut microbial communities associated with AD, w...
Source: Alzheimer's and Dementia: The Journal of the Alzheimer's Association - Category: Geriatrics Source Type: research
In conclusion, our data show how oncogenic and tumor-suppressive drivers of cellular senescence act to regulate surveillance processes that can be circumvented to enable SnCs to elude immune recognition but can be reversed by cell surface-targeted interventions to purge the SnCs that persist in vitro and in patients. Since eliminating SnCs can prevent tumor progression, delay the onset of degenerative diseases, and restore fitness; since NKG2D-Ls are not widely expressed in healthy human tissues and NKG2D-L shedding is an evasion mechanism also employed by tumor cells; and since increasing numbers of B cells express NKG2D ...
Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
ConclusionTo successfully control the outbreak a customized bundled approach was necessary. Current guidelines should be adapted to be suitable for all types of residential care homes and to effectively combat the spread of multi resistant pathogens.
Source: Journal of Hospital Infection - Category: Infectious Diseases Source Type: research
Authors: Roger PM, Lesselingue D, Ferré A, Abram F PMID: 31416654 [PubMed - as supplied by publisher]
Source: Medecine et Maladies Infectieuses - Category: Infectious Diseases Tags: Med Mal Infect Source Type: research
This study was undertaken to highlight the social disparity between rural and urban areas in terms of housing patterns, provision of safe drinking water, access to sanitation facilities, education, employment rate and health-related to diarrhoeal episodes in Ugu District Municipality of KwaZulu-Natal Province of South Africa. To achieve this aim, a survey was conducted using a structured questionnaire. Drinking water samples were collected from the point of supply and the storage containers to assess the microbiological quality of drinking water in both rural and urban areas. Results of this study revealed prominent reside...
Source: International Journal of Environmental Research and Public Health - Category: Environmental Health Authors: Tags: Article Source Type: research
This study reveals the presence of a multigene defensin family in I. holocyclus with wide antimicrobial activity.
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
Authors: Koyuncu Özyurt Ö, Özhak B, Öğünç D, Yıldız E, Çolak D, Günseren F, Öngüt G Abstract Infections with multidrug resistant gram-negative bacteria is a growing problem especially in health care settings. Colistin is one of the last resort antibiotics for such infections in which treatment options are limited. Increasing resistance to colistin is a global problem. Clinical and Laboratory Standards Institute (CLSI) and the European Committee on Antimicrobial Susceptibility Testing (EUCAST) study groups have recommended the ISO-standard broth microdilution ...
Source: Mikrobiyoloji Bulteni - Category: Microbiology Tags: Mikrobiyol Bul Source Type: research
Publication date: Available online 16 August 2019Source: Molecular and Cellular ProbesAuthor(s): Ping Zhou, Guoyang Xie, Taobo Liang, Bei Yu, Zoraida Aguilar, Hengyi XuAbstractEmetic Bacillus cereus is one of the causative agents of foodborne diseases which can cause vomiting-type food poisoning after ingestion of contaminated food. To minimize B. cereus food poisoning, propidium monoazide (PMA) combined with quantitative polymerase chain reaction (qPCR) called PMA-qPCR was applied for detecting viable emetic B. cereus in milk. The cereulide synthetase gene of emetic B. cereus (cesB) was chosen for the primer, and PMA trea...
Source: Molecular and Cellular Probes - Category: Molecular Biology Source Type: research
Publication date: Available online 16 August 2019Source: Protein Expression and PurificationAuthor(s): Marcia A. LeVatte, Matthias Lipfert, Carol Ladner-Keay, David S. WishartAbstractAlzheimer's disease (AD) is a progressive neurological disease marked by the accumulation and deposition of misfolded amyloid beta or Abeta (Aβ) peptide. Two species of Aβ peptides are found in amyloid plaques, Aβ1-40 and Aβ1-42, with the latter being the more amyloidogenic of the two. Understanding how and why Aβ peptides misfold, oligomerize and form amyloid plaques requires a detailed understanding of their structur...
Source: Protein Expression and Purification - Category: Biochemistry Source Type: research
Publication date: Available online 16 August 2019Source: Polymer TestingAuthor(s): Natalia Sienkiewicz, Sylwia CzłonkaAbstractThis paper presents the effect of natural compound (E,E)-1,7-bis(4-Hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione (curcumin) on the antibacterial, anti-aging, mechanical and morphological properties of polyurethane (PU) rigid foams. Concentrations of 1 wt%, 2 wt% and 5 wt% of antibacterial compound was used, and the infection reduction capacity was evaluated for different types of bacteria (Escherichia coli (G-) and Staphylococcus aureus (G+). In order to determine all assumed properties t...
Source: Polymer Testing - Category: Chemistry Source Type: research
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