Microbial production of sialic acid and sialylated human milk oligosaccharides: Advances and perspectives.

Microbial production of sialic acid and sialylated human milk oligosaccharides: Advances and perspectives.
Biotechnol Adv. 2019 Apr 24;:
Authors: Zhang X, Liu Y, Liu L, Li J, Du G, Chen J
Abstract
Sialic acids (SAs) are important functional sugars, and monomers of sialylated human milk oligosaccharides (sialylated HMOs or sialyllactoses), which are crucial for improving infant development and can facilitate infant brain development, maintain brain health, and enhance immunity. The most common form of SA is N-acetylneuraminic acid (NeuAc), and the main forms of sialyllactoses are 6'-sialyllactose (6'-SL) and 3'-sialyllactose (3'-SL). As functional food additive, the demand for NeuAc and sialyllactoses will continuously increase due to their wide and important fields of application. However, NeuAc and sialyllactoses produced by traditional extraction methods are inefficient and may cause allergen contamination, and cannot keep up with the rapidly increasing market demand. Therefore, the production of NeuAc and sialyllactoses by sustainable biotechnological methods have attracted increasing attention. In particular, the development of metabolic engineering and synthetic biology techniques and strategies have promoted efficient biosynthesis of NeuAc and sialyllactoses. In this review, we first discussed the application of NeuAc and sialyllactoses. Secondly, metabolic engineering and protein engineering-fueled progress of whole-cell catal...

Source: Biotechnology Advances - April 23, 2019 Category: Biotechnology Authors: Zhang X, Liu Y, Liu L, Li J, Du G, Chen J Tags: Biotechnol Adv Source Type: research